Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   ACFW99_RS10950 Genome accession   NZ_CP170651
Coordinates   2252234..2252842 (+) Length   202 a.a.
NCBI ID   WP_015417702.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2247234..2257842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS10910 (ACFW99_10910) - 2248286..2248792 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  ACFW99_RS10915 (ACFW99_10915) - 2248931..2249344 (+) 414 WP_039254739.1 Hsp20/alpha crystallin family protein -
  ACFW99_RS10920 (ACFW99_10920) - 2249374..2249808 (-) 435 WP_007409493.1 YppG family protein -
  ACFW99_RS10925 (ACFW99_10925) - 2249995..2250177 (+) 183 WP_003153532.1 YppF family protein -
  ACFW99_RS10930 (ACFW99_10930) - 2250216..2250584 (-) 369 WP_007409492.1 YppE family protein -
  ACFW99_RS10935 (ACFW99_10935) - 2250630..2250875 (-) 246 WP_012117836.1 DUF5446 family protein -
  ACFW99_RS10940 (ACFW99_10940) sspM 2251082..2251186 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  ACFW99_RS10945 (ACFW99_10945) - 2251230..2252192 (-) 963 WP_015417701.1 DUF2515 domain-containing protein -
  ACFW99_RS10950 (ACFW99_10950) recU 2252234..2252842 (+) 609 WP_015417702.1 Holliday junction resolvase RecU Machinery gene
  ACFW99_RS10955 (ACFW99_10955) - 2252881..2255664 (+) 2784 WP_015417703.1 PBP1A family penicillin-binding protein -
  ACFW99_RS10960 (ACFW99_10960) - 2255742..2256233 (-) 492 WP_015417704.1 YpoC family protein -
  ACFW99_RS10965 (ACFW99_10965) nth 2256230..2256889 (-) 660 WP_007612187.1 endonuclease III -
  ACFW99_RS10970 (ACFW99_10970) dnaD 2256908..2257606 (-) 699 WP_015417705.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23675.77 Da        Isoelectric Point: 9.5779

>NTDB_id=1058412 ACFW99_RS10950 WP_015417702.1 2252234..2252842(+) (recU) [Bacillus amyloliquefaciens strain JP5025]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLHNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
NNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=1058412 ACFW99_RS10950 WP_015417702.1 2252234..2252842(+) (recU) [Bacillus amyloliquefaciens strain JP5025]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCCCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCCACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTTGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCATAATTTTCACGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AACAATGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterProScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

89.95

98.515

0.886

Multiple sequence alignment