Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFW99_RS08890 Genome accession   NZ_CP170651
Coordinates   1833072..1833506 (+) Length   144 a.a.
NCBI ID   WP_015417516.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1828072..1838506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS08885 (ACFW99_08885) - 1831572..1832783 (-) 1212 WP_031379100.1 cytochrome P450 -
  ACFW99_RS08890 (ACFW99_08890) nucA/comI 1833072..1833506 (+) 435 WP_015417516.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACFW99_RS08895 (ACFW99_08895) - 1833566..1834321 (+) 756 WP_003154084.1 YoaK family protein -
  ACFW99_RS08900 (ACFW99_08900) - 1834355..1834717 (-) 363 WP_031379099.1 hypothetical protein -
  ACFW99_RS08905 (ACFW99_08905) - 1834909..1836237 (-) 1329 WP_015417518.1 S8 family peptidase -
  ACFW99_RS08910 (ACFW99_08910) - 1836416..1836649 (+) 234 WP_015239897.1 hypothetical protein -
  ACFW99_RS08915 (ACFW99_08915) - 1836905..1837612 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  ACFW99_RS08920 (ACFW99_08920) - 1837672..1838124 (+) 453 WP_014305039.1 OsmC family protein -
  ACFW99_RS08925 (ACFW99_08925) - 1838138..1838491 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15514.44 Da        Isoelectric Point: 7.2418

>NTDB_id=1058411 ACFW99_RS08890 WP_015417516.1 1833072..1833506(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5025]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1058411 ACFW99_RS08890 WP_015417516.1 1833072..1833506(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5025]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCGTGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment