Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ACFW99_RS05640 Genome accession   NZ_CP170651
Coordinates   1122451..1124280 (+) Length   609 a.a.
NCBI ID   WP_007409107.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1117451..1129280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS05615 (ACFW99_05615) - 1118115..1118693 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  ACFW99_RS05620 (ACFW99_05620) spx 1118871..1119266 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  ACFW99_RS05625 (ACFW99_05625) - 1119324..1119980 (-) 657 WP_007409109.1 TerC family protein -
  ACFW99_RS05630 (ACFW99_05630) mecA 1120256..1120912 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  ACFW99_RS05635 (ACFW99_05635) - 1121063..1122223 (+) 1161 WP_061861354.1 competence protein CoiA family protein -
  ACFW99_RS05640 (ACFW99_05640) pepF 1122451..1124280 (+) 1830 WP_007409107.1 oligoendopeptidase F Regulator
  ACFW99_RS05645 (ACFW99_05645) - 1124318..1124485 (-) 168 WP_003155026.1 hypothetical protein -
  ACFW99_RS05650 (ACFW99_05650) spxH 1124771..1125673 (-) 903 WP_039251990.1 protease adaptor protein SpxH -
  ACFW99_RS05655 (ACFW99_05655) - 1125670..1126068 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  ACFW99_RS05660 (ACFW99_05660) - 1126297..1126983 (-) 687 WP_109566857.1 lytic transglycosylase domain-containing protein -
  ACFW99_RS05665 (ACFW99_05665) - 1126988..1127560 (-) 573 WP_061861356.1 CYTH domain-containing protein -
  ACFW99_RS05670 (ACFW99_05670) - 1127685..1128050 (+) 366 WP_012117290.1 hypothetical protein -
  ACFW99_RS05675 (ACFW99_05675) yjbM 1128078..1128713 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69813.45 Da        Isoelectric Point: 5.2024

>NTDB_id=1058401 ACFW99_RS05640 WP_007409107.1 1122451..1124280(+) (pepF) [Bacillus amyloliquefaciens strain JP5025]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDIYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=1058401 ACFW99_RS05640 WP_007409107.1 1122451..1124280(+) (pepF) [Bacillus amyloliquefaciens strain JP5025]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCCTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TGGAAAAAGAGGAGCTTAAACTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGTGTTTTAAATAACGCCGATAT
TACATTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAGTATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTGATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTCGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATATATACGAAAATAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGACAATGTCAATAATCTGTTTACGCTCGTTCATGAGTTCGGCCATTCC
GTGCACAGTTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.832

97.537

0.486

Multiple sequence alignment