Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFU5E_RS01945 Genome accession   NZ_CP170577
Coordinates   430069..431508 (+) Length   479 a.a.
NCBI ID   WP_043140004.1    Uniprot ID   -
Organism   Aeromonas bestiarum strain S1-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425069..436508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFU5E_RS01915 (ACFU5E_01915) mlaF 425646..426446 (-) 801 WP_043140015.1 phospholipid ABC transporter ATP-binding protein MlaF -
  ACFU5E_RS01920 (ACFU5E_01920) - 426654..427649 (+) 996 WP_378081144.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACFU5E_RS01925 (ACFU5E_01925) kdsC 427649..428203 (+) 555 WP_241325653.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACFU5E_RS01930 (ACFU5E_01930) lptC 428200..428760 (+) 561 WP_098981825.1 LPS export ABC transporter periplasmic protein LptC -
  ACFU5E_RS01935 (ACFU5E_01935) lptA 428747..429274 (+) 528 WP_042867839.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFU5E_RS01940 (ACFU5E_01940) lptB 429278..430003 (+) 726 WP_042867841.1 LPS export ABC transporter ATP-binding protein -
  ACFU5E_RS01945 (ACFU5E_01945) rpoN/rpoN1 430069..431508 (+) 1440 WP_043140004.1 RNA polymerase factor sigma-54 Machinery gene
  ACFU5E_RS01950 (ACFU5E_01950) hpf 431530..431817 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  ACFU5E_RS01955 (ACFU5E_01955) ptsN 431820..432266 (+) 447 WP_042867845.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACFU5E_RS01960 (ACFU5E_01960) rapZ 432309..433175 (+) 867 WP_043140001.1 RNase adapter RapZ -
  ACFU5E_RS01965 (ACFU5E_01965) npr 433196..433468 (+) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  ACFU5E_RS01970 (ACFU5E_01970) - 433487..435391 (-) 1905 WP_378081145.1 BglG family transcription antiterminator -
  ACFU5E_RS01975 (ACFU5E_01975) - 435495..436235 (-) 741 WP_378081146.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53807.73 Da        Isoelectric Point: 4.3416

>NTDB_id=1058078 ACFU5E_RS01945 WP_043140004.1 430069..431508(+) (rpoN/rpoN1) [Aeromonas bestiarum strain S1-4]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEVQETSPEEPVDASQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLTEAREVINEHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGTKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1058078 ACFU5E_RS01945 WP_043140004.1 430069..431508(+) (rpoN/rpoN1) [Aeromonas bestiarum strain S1-4]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAACCCCTTGCTGGAGCAAGAGGAGAATGAGG
TGCAGGAGACCAGCCCGGAAGAGCCGGTCGATGCCAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGAGGATGTCTACTCGGCCGGCACGGCCCAGAGCGCAGGCCCCATCCACGACGG
GGAAGACAGCGTCTATCAGGGCGAAACCACCGAGAATCTGCAGGACTATCTGCTGTGGCAGATGCGACTCACGCCGTTCA
GCGATCTGGATGCCGCCATTGCCCTCGCCATCATAGATGCCATCGACGACTCCGGTTATCTGACCGTCTCCCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTAGAACTCGACGAGGTCGAGGCCGTGCTCAAGCGAGTGCAGCATTT
CGATCCAGTCGGCATCGCCGCCCGCTCGGTGCAGGAATGCCTGTTGATCCAGCTCGGTCAATACGCCCCCGAACTGCCCT
GGCTCACCGAGGCCAGAGAGGTGATCAACGAGCACATGGACTTGCTCGGCAATCGCGATTATCGCACCCTGGCGCGCAAG
ACCCGACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCCAGACCGGGCAATGGCATCAT
ACAGAGCGACTCCCAGTACGTGATCCCGGATGTGACCGTGGTCAAGAAGGGCACCAAATGGGTGGTCGAGCTCAATCCCG
ATTCCGCGCCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCACGCCAGGCGCGCACCAGCCAGGACACCCAGTTC
ATCCGTTCCCATCTGCAAGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGCAATGACACCCTGCTCAAGGTGGCCAG
CTGTATTGTCGAGCAGCAGCAGGGCTTTTTCGAGTATGGTGAAGAGGCCATGAAGCCCATGGTGCTCAACGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATTTCCCGGGTAACGACCCAGAAATACATGCATACGCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAATACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCAGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.169

100

0.491

Multiple sequence alignment