Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFJIS_RS06430 Genome accession   NZ_CP170558
Coordinates   1369635..1371224 (-) Length   529 a.a.
NCBI ID   WP_307636062.1    Uniprot ID   A0AAW8DS60
Organism   Variovorax boronicumulans strain UC19_11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1364635..1376224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIS_RS06405 (ACFJIS_06405) - 1365467..1366078 (+) 612 WP_390347032.1 cytochrome b -
  ACFJIS_RS06410 (ACFJIS_06410) - 1366075..1366659 (+) 585 WP_062480316.1 YceI family protein -
  ACFJIS_RS06415 (ACFJIS_06415) - 1366698..1367330 (+) 633 WP_095743357.1 YceI family protein -
  ACFJIS_RS06420 (ACFJIS_06420) - 1367356..1368003 (+) 648 WP_095743356.1 LysE family translocator -
  ACFJIS_RS06425 (ACFJIS_06425) - 1368008..1369624 (-) 1617 WP_390347035.1 phospholipase D family protein -
  ACFJIS_RS06430 (ACFJIS_06430) rpoN/rpoN1 1369635..1371224 (-) 1590 WP_307636062.1 RNA polymerase factor sigma-54 Machinery gene
  ACFJIS_RS06435 (ACFJIS_06435) lptB 1371379..1372173 (-) 795 WP_070063734.1 LPS export ABC transporter ATP-binding protein -
  ACFJIS_RS06440 (ACFJIS_06440) lptA 1372170..1372889 (-) 720 WP_159278745.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFJIS_RS06445 (ACFJIS_06445) - 1373012..1373656 (-) 645 WP_390347038.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACFJIS_RS06450 (ACFJIS_06450) - 1373809..1374576 (-) 768 WP_390347040.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58396.70 Da        Isoelectric Point: 4.6849

>NTDB_id=1057999 ACFJIS_RS06430 WP_307636062.1 1369635..1371224(-) (rpoN/rpoN1) [Variovorax boronicumulans strain UC19_11]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDTADAPVPRDDAGEGEAEF
TPSAASTSSTSSDTTTSTPDTDGPAAEIAEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESLQ
SFLHRQALSLRLNENDCAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPVGVGARDLG
ECLSIQLKALASEDETEAEALIRKTAIAICKQPMELLARRDFKRLATLTRTNEELVRSALQIIARLEPKPGRRFVDVERN
IVIPDVIVTKTGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1057999 ACFJIS_RS06430 WP_307636062.1 1369635..1371224(-) (rpoN/rpoN1) [Variovorax boronicumulans strain UC19_11]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCATCTGGCCTTGACGCCACAGCTGCAGCAGTCCATTCGCCTGCTCCA
GCTCTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CGGCGCGCGAGGAGTTCGGGCTGGACACGGCCGACGCGCCGGTGCCGCGCGACGACGCGGGCGAGGGGGAGGCCGAGTTC
ACGCCGTCCGCCGCCAGCACTTCCTCCACGTCCAGCGACACCACCACCTCGACCCCCGACACCGACGGCCCCGCCGCCGA
GATCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAGTGGGGCAGCGATG
CGCCTGCGCGCCAGAACAACCTGGGCGACGACGAGCGGGCCGATGCCACCGAACTCGCGCGCAGCCAGGAGTCGCTGCAG
TCCTTCCTGCACCGCCAGGCGCTGAGCCTGCGCCTGAACGAAAACGACTGCGCCGCGCTGCGCTTTTTGATCGAATCGCT
CAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCATTGGCCTCGGGCCTGGCGGGCGACGACAACGACCAGTTCGACG
ACCTCGTGCACCACTTCCAGGTGGCGCTGGGCCTGCTGCAGAGCCTGGAGCCGGTGGGCGTCGGCGCGCGCGACCTCGGC
GAATGCCTGAGCATCCAGCTGAAGGCGCTCGCCAGCGAAGACGAGACCGAGGCCGAAGCGCTCATCCGCAAGACAGCCAT
CGCGATCTGCAAGCAGCCCATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCGACGCTCACGCGCACCAACGAAG
AGCTGGTGCGCTCGGCGCTGCAGATCATTGCGCGCCTGGAGCCCAAGCCGGGCCGCCGCTTTGTCGACGTCGAACGCAAC
ATCGTCATTCCCGACGTGATCGTCACCAAGACCGGGCGCGGCACGAACGTGAAGTTCCGCGTCATGCTCAACCCCGAGGT
CATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCCATC
GTCGAGCGCCAGAAGAGCTACTTCGTGCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACGGTCGAGCTCAAGT
ACTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGTGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTC
GTGAGTTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATTTCCGAGATGCTGAAAGAGCAGGGCATCGAGTGCGC
GCGCCGCACCGTCGCCAAGTACCGCGAAGCCTTGCGCATCGCACCGGCCAACCTGCGCAAGGCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.601

100

0.518

Multiple sequence alignment