Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACFJI2_RS01205 Genome accession   NZ_CP170549
Coordinates   263070..264134 (-) Length   354 a.a.
NCBI ID   WP_377703398.1    Uniprot ID   -
Organism   Pseudoduganella sp. UC29_71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 258070..269134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJI2_RS01185 (ACFJI2_01185) - 259578..260021 (-) 444 WP_377703395.1 hypothetical protein -
  ACFJI2_RS01190 (ACFJI2_01190) rpoH 260161..261060 (-) 900 WP_132136798.1 RNA polymerase sigma factor RpoH -
  ACFJI2_RS01195 (ACFJI2_01195) ftsX 261453..262367 (-) 915 WP_377703396.1 permease-like cell division protein FtsX -
  ACFJI2_RS01200 (ACFJI2_01200) - 262364..263047 (-) 684 WP_377703397.1 cell division ATP-binding protein FtsE -
  ACFJI2_RS01205 (ACFJI2_01205) pilA 263070..264134 (-) 1065 WP_377703398.1 signal recognition particle-docking protein FtsY Machinery gene
  ACFJI2_RS01210 (ACFJI2_01210) - 264266..265207 (-) 942 WP_377703399.1 MerR family transcriptional regulator -
  ACFJI2_RS01215 (ACFJI2_01215) - 265969..267045 (+) 1077 WP_377703400.1 porin -
  ACFJI2_RS01220 (ACFJI2_01220) - 267125..267691 (+) 567 WP_377703401.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37360.36 Da        Isoelectric Point: 6.3619

>NTDB_id=1057910 ACFJI2_RS01205 WP_377703398.1 263070..264134(-) (pilA) [Pseudoduganella sp. UC29_71]
MFSFFKKKPVQPETPPVLPTAAAPAPTDAPAPAADAAGTLLPATQAPLTAPGAEQDKPNWMARLKAGLSKTSANLSVLFV
GAKIDDDLYDELEAALLMSDAGIDATEFLLDALKRKVKEDKLLDAAAVKAALKTLMIELLAPLQKPFELGRHQPLVMMIS
GVNGAGKTTTIGKLAKHMQGHGQSVLLAAGDTFRAAAREQLMVWGQRNNVTVISQESGDPAAVSYDAVQSAKAKGIDVVM
IDTAGRLPTQLHLMEELKKVKRVLGKGLEGAPHETLLVIDGNTGQNALAQVKAFDDALQLSGLIITKLDGTAKGGVIAAI
ARVRPVPVYFIGIGERIEDLQPFNATEFVEALLG

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1057910 ACFJI2_RS01205 WP_377703398.1 263070..264134(-) (pilA) [Pseudoduganella sp. UC29_71]
ATGTTTAGCTTCTTCAAGAAAAAACCTGTCCAGCCTGAGACTCCTCCCGTCCTGCCCACGGCAGCCGCCCCCGCGCCAAC
GGACGCCCCGGCGCCTGCCGCCGACGCTGCCGGCACGCTGCTGCCGGCCACGCAAGCCCCATTGACCGCCCCCGGCGCCG
AGCAAGACAAGCCGAACTGGATGGCGCGCCTGAAAGCCGGCCTGTCCAAGACCTCCGCCAACCTGTCCGTGCTGTTTGTC
GGCGCCAAGATCGACGACGACCTGTACGACGAGCTGGAAGCGGCGCTGCTGATGTCCGACGCCGGCATCGACGCCACCGA
ATTCCTGCTCGACGCGCTCAAGCGCAAGGTCAAGGAAGACAAGCTGCTGGACGCGGCTGCCGTGAAGGCCGCGCTGAAAA
CCCTGATGATCGAGCTGCTGGCGCCGCTGCAAAAGCCGTTCGAGCTGGGCCGCCACCAGCCGCTGGTGATGATGATTTCC
GGCGTCAACGGCGCCGGCAAGACCACCACCATCGGCAAGCTGGCCAAGCACATGCAGGGCCACGGCCAGTCGGTGCTGCT
GGCCGCCGGCGACACCTTCCGCGCCGCCGCCCGCGAGCAGCTGATGGTGTGGGGCCAGCGCAACAACGTCACCGTCATCT
CGCAGGAGTCCGGCGACCCGGCCGCCGTGTCCTACGACGCGGTGCAGTCGGCCAAGGCCAAGGGCATCGACGTGGTCATG
ATCGACACCGCCGGCCGCCTGCCGACCCAGCTGCACCTGATGGAAGAGCTGAAAAAGGTCAAGCGCGTGCTGGGCAAGGG
CCTGGAAGGCGCGCCGCACGAAACCCTGCTGGTAATCGACGGCAACACCGGCCAGAACGCACTGGCGCAGGTGAAAGCCT
TCGACGACGCGCTGCAACTGAGCGGCCTCATCATCACCAAGCTGGACGGCACGGCCAAGGGCGGCGTCATCGCGGCCATT
GCCCGCGTGCGCCCGGTGCCGGTGTACTTCATCGGCATCGGCGAGCGCATCGAGGACCTGCAGCCGTTCAACGCCACGGA
ATTCGTCGAAGCCCTGCTCGGATAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(81-133)

SRP54-type protein, GTPase domain (SRP54)

(155-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

55.62

98.023

0.545

Multiple sequence alignment