Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VWC80_RS20490 Genome accession   NZ_CP170495
Coordinates   4006060..4006494 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain IPPBIOTSUC-1012     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4001060..4011494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VWC80_RS20475 (VWC80_20455) - 4002238..4002795 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  VWC80_RS20480 (VWC80_20460) metE 4003307..4005595 (+) 2289 WP_087875691.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  VWC80_RS20485 (VWC80_20465) comJ 4005634..4006023 (-) 390 WP_000424030.1 competence protein ComJ -
  VWC80_RS20490 (VWC80_20470) nucA/comI 4006060..4006494 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  VWC80_RS20495 (VWC80_20475) - 4006721..4007470 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  VWC80_RS20500 (VWC80_20480) kinB 4007509..4008783 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  VWC80_RS20505 (VWC80_20485) - 4009094..4009294 (+) 201 WP_000929277.1 hypothetical protein -
  VWC80_RS20510 (VWC80_20490) - 4009417..4010580 (+) 1164 WP_000434578.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=1057746 VWC80_RS20490 WP_000811494.1 4006060..4006494(-) (nucA/comI) [Bacillus thuringiensis strain IPPBIOTSUC-1012]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1057746 VWC80_RS20490 WP_000811494.1 4006060..4006494(-) (nucA/comI) [Bacillus thuringiensis strain IPPBIOTSUC-1012]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment