Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFDSG_RS14110 Genome accession   NZ_CP170487
Coordinates   2643962..2644384 (-) Length   140 a.a.
NCBI ID   WP_377521444.1    Uniprot ID   -
Organism   Priestia megaterium strain AJ5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2638962..2649384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDSG_RS14080 - 2639082..2639567 (+) 486 WP_241096356.1 hypothetical protein -
  ACFDSG_RS14085 - 2639866..2640411 (-) 546 WP_377521441.1 GNAT family N-acetyltransferase -
  ACFDSG_RS14090 - 2640706..2641986 (+) 1281 WP_377521442.1 glycosyltransferase -
  ACFDSG_RS14095 - 2641958..2642665 (+) 708 WP_377521443.1 TetR/AcrR family transcriptional regulator -
  ACFDSG_RS14100 - 2642820..2643413 (+) 594 WP_028409296.1 hypothetical protein -
  ACFDSG_RS14105 - 2643454..2643798 (-) 345 WP_025751033.1 DUF1516 family protein -
  ACFDSG_RS14110 nucA/comI 2643962..2644384 (-) 423 WP_377521444.1 DNA-entry nuclease Machinery gene
  ACFDSG_RS14115 - 2644520..2644984 (-) 465 WP_128820261.1 FixH family protein -
  ACFDSG_RS14120 - 2645014..2646396 (-) 1383 WP_377521445.1 PepSY-associated TM helix domain-containing protein -
  ACFDSG_RS14125 - 2646548..2647534 (-) 987 WP_047931981.1 glycerophosphodiester phosphodiesterase family protein -
  ACFDSG_RS14130 - 2647712..2648353 (-) 642 WP_034650500.1 response regulator transcription factor -
  ACFDSG_RS14135 - 2648354..2649175 (-) 822 WP_377521446.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15285.12 Da        Isoelectric Point: 4.4990

>NTDB_id=1057642 ACFDSG_RS14110 WP_377521444.1 2643962..2644384(-) (nucA/comI) [Priestia megaterium strain AJ5]
MKKIVSGILILVLAVIVMIEGNPLEENSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEGNRRESLK
GIPTKSGYDRDEFPMAFCEEGGNGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=1057642 ACFDSG_RS14110 WP_377521444.1 2643962..2644384(-) (nucA/comI) [Priestia megaterium strain AJ5]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGGAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGTGCTGAAGGAAACCGCCGTGAGTCTTTAAAG
GGCATTCCGACTAAAAGCGGCTACGATCGAGATGAGTTTCCAATGGCCTTTTGTGAAGAAGGCGGAAACGGTGCAGACAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGATCTTGGATCTCACATCAAGTAGACGAGTTTCCAGACGGCACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.483

82.857

0.493

Multiple sequence alignment