Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFDSG_RS11300 Genome accession   NZ_CP170487
Coordinates   2174020..2174394 (-) Length   124 a.a.
NCBI ID   WP_251568743.1    Uniprot ID   -
Organism   Priestia megaterium strain AJ5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2169020..2179394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDSG_RS11275 - 2169590..2169959 (-) 370 Protein_2127 winged helix-turn-helix transcriptional regulator -
  ACFDSG_RS11280 - 2170202..2170717 (+) 516 WP_016765179.1 enoyl-CoA hydratase/isomerase family protein -
  ACFDSG_RS11285 - 2170857..2170982 (+) 126 WP_016765178.1 hypothetical protein -
  ACFDSG_RS11290 helD 2171227..2173548 (+) 2322 WP_016765177.1 RNA polymerase recycling motor HelD -
  ACFDSG_RS11295 comJ 2173577..2173984 (-) 408 WP_016765176.1 competence protein ComJ -
  ACFDSG_RS11300 nucA/comI 2174020..2174394 (-) 375 WP_251568743.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACFDSG_RS11305 - 2174684..2174944 (+) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  ACFDSG_RS11310 - 2175101..2177050 (+) 1950 WP_258357320.1 FUSC family protein -
  ACFDSG_RS11315 - 2177086..2177904 (-) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  ACFDSG_RS11320 - 2178230..2178901 (+) 672 WP_377521085.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13733.97 Da        Isoelectric Point: 4.2132

>NTDB_id=1057639 ACFDSG_RS11300 WP_251568743.1 2174020..2174394(-) (nucA/comI) [Priestia megaterium strain AJ5]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAEHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=1057639 ACFDSG_RS11300 WP_251568743.1 2174020..2174394(-) (nucA/comI) [Priestia megaterium strain AJ5]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAATTATGATGCGACACTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGAGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGAGAAGAATCCCTGAAAGGATATCCGACAAAAACAGGTTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(68-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.387

95.968

0.637

Multiple sequence alignment