Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R9B26_RS20640 Genome accession   NZ_AP028420
Coordinates   4260573..4262006 (+) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter asburiae strain TA61661     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4255573..4267006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R9B26_RS20610 (EATA6166_39840) - 4256140..4257117 (+) 978 WP_047646430.1 calcium/sodium antiporter -
  R9B26_RS20615 (EATA6166_39850) kdsD 4257131..4258117 (+) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  R9B26_RS20620 (EATA6166_39860) kdsC 4258132..4258698 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  R9B26_RS20625 (EATA6166_39870) lptC 4258695..4259270 (+) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  R9B26_RS20630 (EATA6166_39880) lptA 4259239..4259793 (+) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  R9B26_RS20635 (EATA6166_39890) lptB 4259800..4260525 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  R9B26_RS20640 (EATA6166_39900) rpoN/rpoN1 4260573..4262006 (+) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  R9B26_RS20645 (EATA6166_39910) hpf 4262029..4262316 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  R9B26_RS20650 (EATA6166_39920) ptsN 4262399..4262890 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  R9B26_RS20655 (EATA6166_39930) rapZ 4262936..4263790 (+) 855 WP_014885397.1 RNase adapter RapZ -
  R9B26_RS20660 (EATA6166_39940) npr 4263787..4264059 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  R9B26_RS20665 (EATA6166_39950) mtgA 4264099..4264824 (-) 726 WP_199027539.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  R9B26_RS20670 (EATA6166_39960) elbB 4264821..4265474 (-) 654 WP_199027572.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=105750 R9B26_RS20640 WP_023309360.1 4260573..4262006(+) (rpoN/rpoN1) [Enterobacter asburiae strain TA61661]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=105750 R9B26_RS20640 WP_023309360.1 4260573..4262006(+) (rpoN/rpoN1) [Enterobacter asburiae strain TA61661]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACTCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTAAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAAACCAG
CGAGCCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACAATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCCTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment