Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACFIOT_RS11470 Genome accession   NZ_CP170414
Coordinates   2290125..2291489 (-) Length   454 a.a.
NCBI ID   WP_001085219.1    Uniprot ID   W8U6A9
Organism   Staphylococcus aureus strain t5047     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2285125..2296489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOT_RS11455 cysE 2285945..2286592 (-) 648 WP_001805237.1 serine O-acetyltransferase -
  ACFIOT_RS11460 gltX 2287016..2288470 (-) 1455 WP_054190483.1 glutamate--tRNA ligase -
  ACFIOT_RS11465 - 2289027..2290100 (-) 1074 WP_054190484.1 PIN/TRAM domain-containing protein -
  ACFIOT_RS11470 radA 2290125..2291489 (-) 1365 WP_001085219.1 DNA repair protein RadA Machinery gene
  ACFIOT_RS11475 - 2291973..2294429 (-) 2457 WP_054190485.1 ATP-dependent Clp protease ATP-binding subunit -
  ACFIOT_RS11480 - 2294443..2295450 (-) 1008 WP_054190486.1 protein arginine kinase -
  ACFIOT_RS11485 - 2295440..2296006 (-) 567 WP_054190487.1 UvrB/UvrC motif-containing protein -
  ACFIOT_RS11490 - 2296025..2296486 (-) 462 WP_000551762.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49835.35 Da        Isoelectric Point: 7.0049

>NTDB_id=1056994 ACFIOT_RS11470 WP_001085219.1 2290125..2291489(-) (radA) [Staphylococcus aureus strain t5047]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1056994 ACFIOT_RS11470 WP_001085219.1 2290125..2291489(-) (radA) [Staphylococcus aureus strain t5047]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGAAAATGTCCTAATTG
TGGTGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGCAG
GGAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACGGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGGGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTACTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATCGCTAAACAAATGAACATTGCAACTTTTATAGTGGGCCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACATATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U6A9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.674
  radA Streptococcus pneumoniae Rx1

62.054

98.678

0.612
  radA Streptococcus pneumoniae D39

62.054

98.678

0.612
  radA Streptococcus pneumoniae R6

62.054

98.678

0.612
  radA Streptococcus pneumoniae TIGR4

62.054

98.678

0.612
  radA Streptococcus mitis NCTC 12261

62.054

98.678

0.612
  radA Streptococcus mitis SK321

62.054

98.678

0.612

Multiple sequence alignment