Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   R8569_RS05575 Genome accession   NZ_AP028413
Coordinates   1093986..1094936 (+) Length   316 a.a.
NCBI ID   WP_002866164.1    Uniprot ID   Q9ALT6
Organism   Campylobacter jejuni strain IDH-12448     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1071401..1106973 1093986..1094936 within 0


Gene organization within MGE regions


Location: 1071401..1106973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8569_RS05455 (I12448_10640) pglI 1071401..1072330 (-) 930 WP_002866141.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  R8569_RS05460 (I12448_10650) pglH 1072323..1073402 (-) 1080 WP_072238645.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  R8569_RS05465 (I12448_10660) pglK 1073399..1075093 (-) 1695 WP_002877063.1 ABC-type lipopolysaccharide transporter PglK -
  R8569_RS05470 (I12448_10670) galE 1075087..1076073 (-) 987 WP_002877060.1 UDP-glucose 4-epimerase GalE -
  R8569_RS05475 (I12448_10680) - 1076132..1076926 (-) 795 WP_002877059.1 3'-5' exonuclease -
  R8569_RS05480 (I12448_10690) waaC 1076992..1078020 (+) 1029 WP_002866145.1 lipopolysaccharide heptosyltransferase I -
  R8569_RS05485 (I12448_10700) htrB 1078010..1078888 (+) 879 WP_002801091.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  R8569_RS05490 (I12448_10710) - 1078885..1079586 (+) 702 WP_002877056.1 glycosyltransferase family 2 protein -
  R8569_RS05495 (I12448_10720) rfbA 1079597..1080475 (+) 879 WP_038402204.1 glucose-1-phosphate thymidylyltransferase RfbA -
  R8569_RS05500 (I12448_10730) rfbB 1080475..1081506 (+) 1032 WP_317648096.1 dTDP-glucose 4,6-dehydratase -
  R8569_RS05505 (I12448_10740) wlaRA 1081509..1081931 (+) 423 WP_317648098.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRA -
  R8569_RS05510 (I12448_10750) wlaRB 1081953..1082354 (+) 402 WP_014517057.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRB -
  R8569_RS05515 (I12448_10760) - 1082351..1083202 (+) 852 WP_014517058.1 formyltransferase family protein -
  R8569_RS05520 (I12448_10770) - 1083199..1083924 (+) 726 WP_317648099.1 class I SAM-dependent methyltransferase -
  R8569_RS05525 (I12448_10780) - 1083950..1084876 (+) 927 WP_014517060.1 hypothetical protein -
  R8569_RS05530 (I12448_10790) - 1084873..1085937 (+) 1065 WP_317648100.1 glycosyltransferase family 2 protein -
  R8569_RS05535 (I12448_10800) - 1085950..1087002 (+) 1053 WP_002869601.1 glycosyltransferase family 2 protein -
  R8569_RS05540 (I12448_10810) wlaRG 1086968..1088053 (+) 1086 WP_025998025.1 class E lipooligosaccharide biosynthesis aminotransferase WlaRG -
  R8569_RS05545 (I12448_10820) - 1088080..1089339 (-) 1260 WP_317648102.1 DUF2972 domain-containing protein -
  R8569_RS05550 (I12448_10840) - 1089336..1090357 (-) 1022 Protein_1076 hypothetical protein -
  R8569_RS05555 (I12448_10850) - 1090428..1091201 (+) 774 WP_002866160.1 glycosyltransferase family 25 protein -
  R8569_RS05560 (I12448_10860) wlaTB 1091194..1092150 (+) 957 WP_002860237.1 class E lipooligosaccharide biosynthesis protein WlaTB -
  R8569_RS05565 (I12448_10870) wlaTC 1092147..1093106 (+) 960 WP_002860238.1 class E lipooligosaccharide biosynthesis protein WlaTC -
  R8569_RS05570 (I12448_10880) - 1093109..1093927 (-) 819 WP_002876193.1 glycosyltransferase family 2 protein -
  R8569_RS05575 (I12448_10890) waaF 1093986..1094936 (+) 951 WP_002866164.1 lipopolysaccharide heptosyltransferase II Regulator
  R8569_RS05580 (I12448_10900) - 1094988..1096487 (+) 1500 WP_002877116.1 MBOAT family O-acyltransferase -
  R8569_RS05585 (I12448_10910) - 1096635..1097612 (+) 978 WP_002866168.1 hypothetical protein -
  R8569_RS05590 (I12448_10920) gmhA 1097616..1098176 (-) 561 WP_002877119.1 D-sedoheptulose 7-phosphate isomerase -
  R8569_RS05595 (I12448_10930) rfaE1 1098173..1099558 (-) 1386 WP_002877121.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  R8569_RS05600 (I12448_10940) rfaD 1099551..1100504 (-) 954 WP_002877123.1 ADP-glyceromanno-heptose 6-epimerase -
  R8569_RS05605 (I12448_10950) gmhB 1100505..1101065 (-) 561 WP_002877124.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  R8569_RS05610 (I12448_10960) - 1101147..1101449 (+) 303 WP_002852762.1 c-type cytochrome -
  R8569_RS05615 (I12448_10970) ccoS 1101472..1101678 (-) 207 WP_002877126.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  R8569_RS05620 (I12448_10980) - 1101675..1104032 (-) 2358 WP_072238644.1 heavy metal translocating P-type ATPase -
  R8569_RS05625 (I12448_10990) rho 1104142..1105440 (+) 1299 WP_002853705.1 transcription termination factor Rho -
  R8569_RS05630 (I12448_11000) dnaX 1105444..1106973 (+) 1530 WP_002877127.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36378.95 Da        Isoelectric Point: 10.3102

>NTDB_id=105653 R8569_RS05575 WP_002866164.1 1093986..1094936(+) (waaF) [Campylobacter jejuni strain IDH-12448]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNTQFILYGSLVSTALFREFPNSKIIIENKQTRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISTVYKIKTVAIFGPTKFTQTSPWQNQNAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEEIKKLSTP

Nucleotide


Download         Length: 951 bp        

>NTDB_id=105653 R8569_RS05575 WP_002866164.1 1093986..1094936(+) (waaF) [Campylobacter jejuni strain IDH-12448]
ATGAAAATTTTTATACATCTTCCCACTTGGCTGGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATACCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAGGGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAAACCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTCAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACATAAGTACGGTTTATAAGATAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATCAAAACGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGTCCTTTAAAGC
ACCATAAATGCATGAAAGATCTAAAGCCTGAAAAGGTGATAGAAGAAATTAAAAAATTAAGCACTCCATAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9ALT6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

94.249

99.051

0.934

Multiple sequence alignment