Detailed information NTG1056154

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: ACFBUK_RS12160
• Genome accession: NZ_CP170331
• Coordinates: 2516031..2518382 (-)
• Length: 783 a.a.
• NCBI ID: WP_032873958.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GDMCC 820630
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2511031..2523382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFBUK_RS12125 (ACFBUK_12125)	-	2511397..2511735 (-)	339	WP_007408270.1	YqxA family protein	-
ACFBUK_RS12130 (ACFBUK_12130)	spoIIP	2511752..2512945 (-)	1194	WP_032873961.1	stage II sporulation protein P	-
ACFBUK_RS12135 (ACFBUK_12135)	gpr	2513013..2514119 (-)	1107	WP_007408268.1	GPR endopeptidase	-
ACFBUK_RS12140 (ACFBUK_12140)	rpsT	2514322..2514588 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACFBUK_RS12145 (ACFBUK_12145)	holA	2514605..2515646 (-)	1042	Protein_2344	DNA polymerase III subunit delta	-
ACFBUK_RS12150 (ACFBUK_12150)	-	2515686..2515837 (-)	152	Protein_2345	hypothetical protein	-
ACFBUK_RS12155 (ACFBUK_12155)	-	2515878..2516012 (+)	135	WP_003152870.1	YqzM family protein	-
ACFBUK_RS12160 (ACFBUK_12160)	comEC	2516031..2518382 (-)	2352	WP_032873958.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFBUK_RS12165 (ACFBUK_12165)	-	2518383..2518952 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACFBUK_RS12170 (ACFBUK_12170)	comEA	2519019..2519633 (-)	615	WP_032873955.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACFBUK_RS12175 (ACFBUK_12175)	comER	2519692..2520513 (+)	822	WP_012118027.1	late competence protein ComER	-
ACFBUK_RS12180 (ACFBUK_12180)	-	2520582..2521319 (-)	738	WP_032873951.1	class I SAM-dependent methyltransferase	-
ACFBUK_RS12185 (ACFBUK_12185)	rsfS	2521316..2521672 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACFBUK_RS12190 (ACFBUK_12190)	yqeK	2521676..2522251 (-)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFBUK_RS12195 (ACFBUK_12195)	-	2522241..2522810 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACFBUK_RS12200 (ACFBUK_12200)	yhbY	2522821..2523111 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86681.60 Da
• Isoelectric Point: 9.1106

>NTDB_id=1056154 ACFBUK_RS12160 WP_032873958.1 2516031..2518382(-) (comEC) [Bacillus velezensis strain GDMCC 820630]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKHHAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLHSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGEKFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLMFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFLMNMVLVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVISYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKVYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=1056154 ACFBUK_RS12160 WP_032873958.1 2516031..2518382(-) (comEC) [Bacillus velezensis strain GDMCC 820630]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCACCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTTATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTCACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAAAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGATGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCTGATGAATATGGTGTTGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTTATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGATTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCAGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTTCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCTACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGTGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.606	98.595	0.558