Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACFDA3_RS10780 Genome accession   NZ_CP170251
Coordinates   2294011..2295384 (-) Length   457 a.a.
NCBI ID   WP_002458651.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain PS08     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2289011..2300384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDA3_RS10765 (ACFDA3_10760) cysE 2290021..2290650 (-) 630 WP_172952487.1 serine O-acetyltransferase -
  ACFDA3_RS10770 (ACFDA3_10765) gltX 2291034..2292488 (-) 1455 WP_049386386.1 glutamate--tRNA ligase -
  ACFDA3_RS10775 (ACFDA3_10770) - 2292899..2293957 (-) 1059 WP_002486699.1 PIN/TRAM domain-containing protein -
  ACFDA3_RS10780 (ACFDA3_10775) radA 2294011..2295384 (-) 1374 WP_002458651.1 DNA repair protein RadA Machinery gene
  ACFDA3_RS10785 (ACFDA3_10780) - 2296091..2298544 (-) 2454 WP_381410267.1 ATP-dependent Clp protease ATP-binding subunit -
  ACFDA3_RS10790 (ACFDA3_10785) - 2298558..2299565 (-) 1008 WP_080033635.1 protein arginine kinase -
  ACFDA3_RS10795 (ACFDA3_10790) - 2299555..2300118 (-) 564 WP_002475503.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50242.84 Da        Isoelectric Point: 7.5278

>NTDB_id=1056084 ACFDA3_RS10780 WP_002458651.1 2294011..2295384(-) (radA) [Staphylococcus epidermidis strain PS08]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKVAHPKHGVKTKETAGKVQKLNSVKHEATPRLLTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICAALSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
EERPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDQAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPQTNIGGWTFPEGIQVVGVSSVHEALKYALHSKPR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1056084 ACFDA3_RS10780 WP_002458651.1 2294011..2295384(-) (radA) [Staphylococcus epidermidis strain PS08]
ATGGCCAAGAAAAAAGTAATATTCGAATGTATGGCATGTGGCTATCAATCTCCTAAGTGGATGGGTAAGTGCCCTAACTG
CGGCGCGTGGAATCAAATGGAAGAAATAGTAGAAAAGGTTGCTCATCCTAAACATGGTGTAAAAACAAAAGAAACAGCAG
GTAAGGTACAAAAGTTAAATAGTGTTAAGCATGAAGCTACCCCTAGACTACTTACAGAATCAAAGGAATTTAACCGCGTT
TTAGGTGGAGGTATTGTAAGTGGTTCATTAGTACTAATAGGTGGAGACCCAGGTATTGGTAAGTCGACTTTATTGCTACA
AATTTGTGCTGCGCTGTCACAAAATAAGAATGTTTTATATATTACTGGTGAGGAATCTATTAATCAAACTAAGTTGCGCG
CTGATCGTTTGGAAGAAGATTCTAGTCATCTTAATGTTTTAGCCGAAACTGATTTAGAAGTGATACATCAAACTGTAAAA
GAAGAGAGACCTGACTTACTTGTTGTTGATTCGATTCAAACAATCTATCATCCGGAAATTAGTTCTGCACCTGGATCGGT
ATCACAAGTAAGAGAGAGTACACAGAGTTTAATGAACATTGCTAAACAGATGAATATTGCCACATTTATTGTGGGACACG
TAACAAAAGAAGGACAAATCGCCGGACCAAGATTATTGGAACATATGGTTGATACAGTTCTTTATTTTGAAGGAGACGAG
CATCACGCTTATCGTATCCTTAGAGCAGTAAAAAATAGATTTGGTTCTACAAATGAGATGGGGATTTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTACTTAATCCTTCTGAAATGTTTTTAGAGGAACGTTCTACAAATGTTCCGGGCTCTACAATCG
TCCCCACTATGGAAGGAACAAGACCACTACTCATTGAAGTCCAAGCGCTTGTTACACCAACTACATTTAATAATCCTAGA
CGAATGGCTACAGGTATAGATCATAATCGATTAAGTTTACTTATGGCGGTTCTAGAAAAAAAGGAAAACTATTTACTCCA
ACAACAAGATGCCTATATTAAAGTAGCAGGTGGCGTCAAATTAACAGAACCTGCTGTTGATTTAAGTATTATTGTTGCGA
CAGCATCAAGTTTTAAAGACCAAGCTGTTGATGGATTAGATTGTTTTGTGGGTGAAGTTGGATTAACAGGTGAAGTACGC
AGAGTATCTCGCATAGAGCAACGTGTTCAAGAAGCGGCCAAACTAGGGTTTAAAAGAGCTATTATTCCACAGACAAATAT
TGGAGGTTGGACATTCCCAGAAGGCATCCAAGTCGTTGGTGTTTCATCAGTACATGAAGCTTTAAAATATGCATTACATT
CAAAACCGAGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.231

100

0.665
  radA Streptococcus pneumoniae Rx1

60.802

98.249

0.597
  radA Streptococcus pneumoniae D39

60.802

98.249

0.597
  radA Streptococcus pneumoniae R6

60.802

98.249

0.597
  radA Streptococcus pneumoniae TIGR4

60.802

98.249

0.597
  radA Streptococcus mitis NCTC 12261

60.802

98.249

0.597
  radA Streptococcus mitis SK321

60.802

98.249

0.597

Multiple sequence alignment