Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACFDHT_RS12515 Genome accession   NZ_CP170227
Coordinates   2536010..2537383 (-) Length   457 a.a.
NCBI ID   WP_039647060.1    Uniprot ID   A0A0A8HTB6
Organism   Staphylococcus hyicus strain SC368     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2531010..2542383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDHT_RS12490 (ACFDHT_12485) cysE 2531266..2531892 (-) 627 WP_168429109.1 serine O-acetyltransferase -
  ACFDHT_RS12495 (ACFDHT_12490) gltX 2532222..2533676 (-) 1455 WP_167694019.1 glutamate--tRNA ligase -
  ACFDHT_RS12500 (ACFDHT_12495) ispF 2533765..2534241 (-) 477 WP_305850401.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACFDHT_RS12505 (ACFDHT_12500) ispD 2534234..2534929 (-) 696 WP_305850402.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACFDHT_RS12510 (ACFDHT_12505) - 2534932..2535993 (-) 1062 WP_039647058.1 PIN/TRAM domain-containing protein -
  ACFDHT_RS12515 (ACFDHT_12510) radA 2536010..2537383 (-) 1374 WP_039647060.1 DNA repair protein RadA Machinery gene
  ACFDHT_RS12520 (ACFDHT_12515) - 2537586..2540063 (-) 2478 WP_039647062.1 ATP-dependent Clp protease ATP-binding subunit -
  ACFDHT_RS12525 (ACFDHT_12520) - 2540076..2541080 (-) 1005 WP_082021559.1 protein arginine kinase -
  ACFDHT_RS12530 (ACFDHT_12525) - 2541073..2541594 (-) 522 WP_039647066.1 UvrB/UvrC motif-containing protein -
  ACFDHT_RS12535 (ACFDHT_12530) - 2541606..2542067 (-) 462 WP_039647067.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50315.98 Da        Isoelectric Point: 8.0136

>NTDB_id=1055728 ACFDHT_RS12515 WP_039647060.1 2536010..2537383(-) (radA) [Staphylococcus hyicus strain SC368]
MAKKKITFECTACGYQSPKWMGKCPNCGAWNSMEESLEQKVAHPKHGVRTQKSTHAKVQTLNEIKNEKTPRILTNSDEFN
RVLGGGIVQGSLVLIGGDPGIGKSTLLLQMCAALSKKKKVLYITGEESLNQTKLRADRLDEDASALNVFAETDLEVIHEA
VKKVEPELIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQAGLKSVPNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIIIATASSFKDQAVDGLDCFIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWTFPENIEVIGVTSTHEALKYALKQR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1055728 ACFDHT_RS12515 WP_039647060.1 2536010..2537383(-) (radA) [Staphylococcus hyicus strain SC368]
GTGGCCAAAAAAAAGATTACATTTGAGTGTACTGCATGCGGATATCAGTCTCCAAAATGGATGGGGAAATGTCCAAATTG
CGGTGCATGGAATTCAATGGAGGAATCATTAGAACAAAAAGTAGCACATCCAAAACATGGTGTGCGTACACAAAAATCGA
CACATGCGAAAGTACAAACGTTAAATGAAATTAAAAATGAAAAAACACCACGCATTCTTACAAATAGTGATGAGTTTAAT
CGTGTACTTGGAGGCGGTATTGTTCAGGGTTCTCTCGTATTGATCGGTGGCGACCCAGGTATCGGTAAATCCACACTACT
CCTTCAAATGTGCGCTGCGTTATCAAAGAAGAAAAAAGTGCTTTATATTACTGGCGAAGAATCACTGAACCAAACCAAAT
TACGTGCAGACCGTTTGGATGAAGATGCCAGTGCGTTGAATGTATTTGCAGAAACAGACCTTGAAGTGATTCACGAGGCA
GTTAAAAAAGTAGAACCTGAGTTAATCGTCGTGGACTCTATTCAAACGATATACCATCCTGAAATTAGTTCTGCACCAGG
ATCGGTGTCCCAAGTGAGAGAAAGTACTCAAAGTCTGATGCATATTGCGAAACAAATGAATATCGCCACATTTATAGTTG
GGCATGTGACAAAAGAAGGTCAAATCGCTGGACCGAGATTGTTAGAACATATGGTGGATACGGTGTTGTATTTCGAAGGA
GACGAACATCATGCGTATCGTATTTTACGTGCCGTTAAAAATCGTTTTGGTTCAACGAATGAAATGGGTATTTTTGAAAT
GAAGCAAGCTGGTTTAAAAAGTGTTCCAAATCCCTCTGAAATGTTTTTAGAAGAGCGTTCAACGAATGTGCCAGGGTCGA
CGATTGTTGCGACAATGGAAGGGACACGCCCTTTACTTATTGAAGTGCAAGCGTTAGTTACACCTACAACATTTAACAAC
CCAAGACGCATGGCCACAGGGATAGATCATAACCGTCTTAGTTTATTAATGGCTGTGTTAGAGAAAAAAGAGGGGTATTT
ATTACAACAACAGGATGCATATATTAAAGTTGCAGGTGGTGTCAAATTAAGTGAGCCCGCTGTGGATTTAGGTATCATTA
TTGCGACAGCTTCAAGTTTTAAAGATCAAGCAGTTGACGGATTAGATTGTTTTATTGGTGAAGTTGGCTTAACTGGCGAA
GTACGCAGAGTGTCACGTATCGAGCAACGTGTACAAGAAGCGGCGAAGTTAGGTTTTAAACGTGTCATTATCCCAAAAAC
CAATATCGGTGGATGGACATTTCCAGAAAATATAGAAGTTATCGGTGTGACTTCAACACATGAAGCTTTAAAATATGCCT
TAAAACAGAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

AAA domain (AAA_25)

(68-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8HTB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.228

99.781

0.661
  radA Streptococcus pneumoniae Rx1

59.867

98.687

0.591
  radA Streptococcus pneumoniae D39

59.867

98.687

0.591
  radA Streptococcus pneumoniae R6

59.867

98.687

0.591
  radA Streptococcus pneumoniae TIGR4

59.867

98.687

0.591
  radA Streptococcus mitis NCTC 12261

59.867

98.687

0.591
  radA Streptococcus mitis SK321

59.867

98.687

0.591

Multiple sequence alignment