Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACFDHU_RS00085 Genome accession   NZ_CP170225
Coordinates   22070..23443 (+) Length   457 a.a.
NCBI ID   WP_039647060.1    Uniprot ID   A0A0A8HTB6
Organism   Staphylococcus hyicus strain SC373     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 17070..28443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDHU_RS00065 (ACFDHU_00065) - 17386..17847 (+) 462 WP_039647067.1 CtsR family transcriptional regulator -
  ACFDHU_RS00070 (ACFDHU_00070) - 17859..18380 (+) 522 WP_039647066.1 UvrB/UvrC motif-containing protein -
  ACFDHU_RS00075 (ACFDHU_00075) - 18373..19377 (+) 1005 WP_082021559.1 protein arginine kinase -
  ACFDHU_RS00080 (ACFDHU_00080) - 19390..21867 (+) 2478 WP_039647062.1 ATP-dependent Clp protease ATP-binding subunit -
  ACFDHU_RS00085 (ACFDHU_00085) radA 22070..23443 (+) 1374 WP_039647060.1 DNA repair protein RadA Machinery gene
  ACFDHU_RS00090 (ACFDHU_00090) - 23460..24521 (+) 1062 WP_039647058.1 PIN/TRAM domain-containing protein -
  ACFDHU_RS00095 (ACFDHU_00095) ispD 24524..25219 (+) 696 WP_305850402.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACFDHU_RS00100 (ACFDHU_00100) ispF 25212..25688 (+) 477 WP_305850401.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACFDHU_RS00105 (ACFDHU_00105) gltX 25777..27231 (+) 1455 WP_167694019.1 glutamate--tRNA ligase -
  ACFDHU_RS00110 (ACFDHU_00110) cysE 27561..28187 (+) 627 WP_168429109.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50315.98 Da        Isoelectric Point: 8.0136

>NTDB_id=1055669 ACFDHU_RS00085 WP_039647060.1 22070..23443(+) (radA) [Staphylococcus hyicus strain SC373]
MAKKKITFECTACGYQSPKWMGKCPNCGAWNSMEESLEQKVAHPKHGVRTQKSTHAKVQTLNEIKNEKTPRILTNSDEFN
RVLGGGIVQGSLVLIGGDPGIGKSTLLLQMCAALSKKKKVLYITGEESLNQTKLRADRLDEDASALNVFAETDLEVIHEA
VKKVEPELIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQAGLKSVPNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIIIATASSFKDQAVDGLDCFIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWTFPENIEVIGVTSTHEALKYALKQR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1055669 ACFDHU_RS00085 WP_039647060.1 22070..23443(+) (radA) [Staphylococcus hyicus strain SC373]
GTGGCCAAAAAAAAGATTACATTTGAGTGTACTGCATGCGGATATCAGTCTCCAAAATGGATGGGGAAATGTCCAAATTG
CGGTGCATGGAATTCAATGGAGGAATCATTAGAACAAAAAGTAGCACATCCAAAACATGGTGTGCGTACACAAAAATCGA
CACATGCGAAAGTACAAACGTTAAATGAAATTAAAAATGAAAAAACACCACGCATTCTTACAAATAGTGATGAGTTTAAT
CGTGTACTTGGAGGCGGTATTGTTCAGGGTTCTCTCGTATTGATCGGTGGCGACCCAGGTATCGGTAAATCCACACTACT
CCTTCAAATGTGCGCTGCGTTATCAAAGAAGAAAAAAGTGCTTTATATTACTGGCGAAGAATCACTGAACCAAACCAAAT
TACGTGCAGACCGTTTGGATGAAGATGCCAGTGCGTTGAATGTATTTGCAGAAACAGACCTTGAAGTGATTCACGAGGCA
GTTAAAAAAGTAGAACCTGAGTTAATCGTCGTGGACTCTATTCAAACGATATACCATCCTGAAATTAGTTCTGCACCAGG
ATCGGTGTCCCAAGTGAGAGAAAGTACTCAAAGTCTGATGCATATTGCGAAACAAATGAATATCGCCACATTTATAGTTG
GGCATGTGACAAAAGAAGGTCAAATCGCTGGACCGAGATTGTTAGAACATATGGTGGATACGGTGTTGTATTTCGAAGGA
GACGAACATCATGCGTATCGTATTTTACGTGCCGTTAAAAATCGTTTTGGTTCAACGAATGAAATGGGTATTTTTGAAAT
GAAGCAAGCTGGTTTAAAAAGTGTTCCAAATCCCTCTGAAATGTTTTTAGAAGAGCGTTCAACGAATGTGCCAGGGTCGA
CGATTGTTGCGACAATGGAAGGGACACGCCCTTTACTTATTGAAGTGCAAGCGTTAGTTACACCTACAACATTTAACAAC
CCAAGACGCATGGCCACAGGGATAGATCATAACCGTCTTAGTTTATTAATGGCTGTGTTAGAGAAAAAAGAGGGGTATTT
ATTACAACAACAGGATGCATATATTAAAGTTGCAGGTGGTGTCAAATTAAGTGAGCCCGCTGTGGATTTAGGTATCATTA
TTGCGACAGCTTCAAGTTTTAAAGATCAAGCAGTTGACGGATTAGATTGTTTTATTGGTGAAGTTGGCTTAACTGGCGAA
GTACGCAGAGTGTCACGTATCGAGCAACGTGTACAAGAAGCGGCGAAGTTAGGTTTTAAACGTGTCATTATCCCAAAAAC
CAATATCGGTGGATGGACATTTCCAGAAAATATAGAAGTTATCGGTGTGACTTCAACACATGAAGCTTTAAAATATGCCT
TAAAACAGAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

AAA domain (AAA_25)

(68-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8HTB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.228

99.781

0.661
  radA Streptococcus pneumoniae Rx1

59.867

98.687

0.591
  radA Streptococcus pneumoniae D39

59.867

98.687

0.591
  radA Streptococcus pneumoniae R6

59.867

98.687

0.591
  radA Streptococcus pneumoniae TIGR4

59.867

98.687

0.591
  radA Streptococcus mitis NCTC 12261

59.867

98.687

0.591
  radA Streptococcus mitis SK321

59.867

98.687

0.591

Multiple sequence alignment