Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACFDHY_RS00220 Genome accession   NZ_CP170216
Coordinates   22248..23621 (+) Length   457 a.a.
NCBI ID   WP_039647060.1    Uniprot ID   A0A0A8HTB6
Organism   Staphylococcus hyicus strain SC393     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 17248..28621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDHY_RS00200 (ACFDHY_00200) - 17563..18024 (+) 462 WP_039647067.1 CtsR family transcriptional regulator -
  ACFDHY_RS00205 (ACFDHY_00205) - 18036..18557 (+) 522 WP_039647066.1 UvrB/UvrC motif-containing protein -
  ACFDHY_RS00210 (ACFDHY_00210) - 18547..19554 (+) 1008 WP_381419900.1 protein arginine kinase -
  ACFDHY_RS00215 (ACFDHY_00215) - 19567..22044 (+) 2478 WP_119635492.1 ATP-dependent Clp protease ATP-binding subunit -
  ACFDHY_RS00220 (ACFDHY_00220) radA 22248..23621 (+) 1374 WP_039647060.1 DNA repair protein RadA Machinery gene
  ACFDHY_RS00225 (ACFDHY_00225) - 23638..24699 (+) 1062 WP_039647058.1 PIN/TRAM domain-containing protein -
  ACFDHY_RS00230 (ACFDHY_00230) ispD 24702..25397 (+) 696 WP_107632718.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACFDHY_RS00235 (ACFDHY_00235) ispF 25390..25866 (+) 477 WP_107632717.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACFDHY_RS00240 (ACFDHY_00240) gltX 25955..27409 (+) 1455 WP_341776353.1 glutamate--tRNA ligase -
  ACFDHY_RS00245 (ACFDHY_00245) cysE 27739..28365 (+) 627 WP_168429109.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50315.98 Da        Isoelectric Point: 8.0136

>NTDB_id=1055543 ACFDHY_RS00220 WP_039647060.1 22248..23621(+) (radA) [Staphylococcus hyicus strain SC393]
MAKKKITFECTACGYQSPKWMGKCPNCGAWNSMEESLEQKVAHPKHGVRTQKSTHAKVQTLNEIKNEKTPRILTNSDEFN
RVLGGGIVQGSLVLIGGDPGIGKSTLLLQMCAALSKKKKVLYITGEESLNQTKLRADRLDEDASALNVFAETDLEVIHEA
VKKVEPELIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQAGLKSVPNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIIIATASSFKDQAVDGLDCFIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWTFPENIEVIGVTSTHEALKYALKQR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1055543 ACFDHY_RS00220 WP_039647060.1 22248..23621(+) (radA) [Staphylococcus hyicus strain SC393]
GTGGCCAAAAAAAAGATTACATTTGAGTGTACTGCATGCGGATATCAGTCTCCAAAATGGATGGGGAAATGTCCAAATTG
CGGTGCATGGAATTCAATGGAGGAATCATTAGAACAAAAAGTAGCACATCCAAAACATGGTGTGCGTACACAAAAATCGA
CACATGCGAAAGTACAAACGTTAAATGAAATTAAAAATGAAAAAACACCACGCATTCTTACAAATAGTGATGAGTTTAAT
CGTGTACTTGGAGGCGGTATTGTTCAGGGTTCTCTCGTATTGATCGGTGGCGACCCAGGTATCGGTAAATCCACACTACT
CCTTCAAATGTGCGCTGCGTTATCAAAGAAGAAAAAAGTGCTTTATATTACTGGCGAAGAATCACTGAACCAAACCAAAT
TACGTGCAGACCGTTTGGATGAAGATGCCAGTGCATTGAATGTATTTGCAGAAACAGACCTTGAAGTGATTCACGAGGCA
GTTAAAAAAGTAGAACCTGAGTTAATCGTCGTGGACTCTATTCAAACGATATACCATCCTGAAATTAGTTCTGCACCAGG
ATCGGTGTCCCAAGTGAGAGAAAGTACTCAAAGTCTGATGCATATTGCGAAACAAATGAATATCGCCACATTTATAGTTG
GGCATGTGACAAAAGAAGGTCAAATCGCTGGACCGAGATTGTTAGAACATATGGTGGATACGGTGTTGTATTTCGAAGGA
GACGAACATCATGCGTATCGTATTTTACGTGCCGTTAAAAATCGTTTTGGTTCAACGAATGAAATGGGTATTTTTGAAAT
GAAGCAAGCTGGTTTAAAAAGTGTTCCAAATCCCTCTGAAATGTTTTTAGAAGAGCGTTCAACGAATGTGCCAGGGTCGA
CGATTGTTGCGACAATGGAAGGGACACGCCCTTTACTTATTGAAGTGCAAGCGTTAGTTACACCTACAACATTTAACAAC
CCAAGACGCATGGCCACAGGGATAGATCATAACCGTCTTAGTTTATTAATGGCTGTGTTAGAGAAAAAAGAGGGGTATTT
ATTACAACAACAGGATGCATATATTAAAGTTGCAGGTGGTGTCAAATTAAGTGAGCCCGCTGTGGATTTAGGTATCATTA
TTGCGACAGCTTCAAGTTTTAAAGATCAAGCAGTTGACGGATTAGATTGTTTTATTGGTGAAGTTGGCTTAACTGGCGAA
GTACGCAGAGTGTCACGTATCGAGCAACGTGTACAAGAAGCGGCGAAGTTAGGTTTTAAACGTGTCATTATCCCAAAAAC
CAATATCGGTGGATGGACATTTCCAGAAAATATAGAAGTTATCGGTGTGACTTCAACACATGAAGCTTTAAAATATGCCT
TAAAACAGAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

AAA domain (AAA_25)

(68-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8HTB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.228

99.781

0.661
  radA Streptococcus pneumoniae Rx1

59.867

98.687

0.591
  radA Streptococcus pneumoniae D39

59.867

98.687

0.591
  radA Streptococcus pneumoniae R6

59.867

98.687

0.591
  radA Streptococcus pneumoniae TIGR4

59.867

98.687

0.591
  radA Streptococcus mitis NCTC 12261

59.867

98.687

0.591
  radA Streptococcus mitis SK321

59.867

98.687

0.591

Multiple sequence alignment