Detailed information NTG0105552

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: DI45_RS07350
• Genome accession: NZ_CP007560
• Coordinates: 1416624..1419008 (-)
• Length: 794 a.a.
• NCBI ID: WP_002988956.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NGAS743
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1411624..1424008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DI45_RS07330 (DI45_07620)	pknB	1411642..1413540 (-)	1899	WP_012560855.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
DI45_RS07335 (DI45_07625)	-	1413537..1414277 (-)	741	WP_002983660.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
DI45_RS07340 (DI45_07630)	rsmB	1414315..1415637 (-)	1323	WP_002983657.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
DI45_RS07345 (DI45_07635)	fmt	1415627..1416562 (-)	936	WP_012560856.1	methionyl-tRNA formyltransferase	-
DI45_RS07350 (DI45_07640)	priA	1416624..1419008 (-)	2385	WP_002988956.1	primosomal protein N'	Machinery gene
DI45_RS07355 (DI45_07645)	rpoZ	1419073..1419390 (-)	318	WP_002983650.1	DNA-directed RNA polymerase subunit omega	-
DI45_RS07360 (DI45_07650)	gmk	1419406..1420041 (-)	636	WP_002983649.1	guanylate kinase	-
DI45_RS07365 (DI45_07655)	-	1420151..1421758 (-)	1608	WP_002988954.1	ribonuclease Y	-
DI45_RS07370 (DI45_07660)	-	1421888..1422784 (-)	897	WP_047235423.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90397.39 Da
• Isoelectric Point: 9.4590

>NTDB_id=105552 DI45_RS07350 WP_002988956.1 1416624..1419008(-) (priA) [Streptococcus pyogenes strain NGAS743]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVAYFVTNHLIRIDERQI
DRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSDNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=105552 DI45_RS07350 WP_002988956.1 1416624..1419008(-) (priA) [Streptococcus pyogenes strain NGAS743]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTGTTA
TTGGTTTTGGCCAGGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAA
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTTTAAAAAAGCCTGATAGAGACTTTCTTTTTGAAGGAA
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGAAAAAATAGCTCTTAAGGCCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTCGAGATGTGGTGGCTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCAAATT
GATCGCAGTGAGCCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAAGCGTCAGCTGTTAC
TGAAATTGTTGAGCAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCAGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTACCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATTGATGAGGAGCATGAATCAACCTATAAACAAGAAAGCAACCCTAGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGGGTCTATCATTTTATTCAATTAACGCAAAGAGCAAATCCTCTTGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGTAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGTTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCCTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AACCTATCCCAATTACTTGTCCTGAGTGTCATAGCAACAGTATCCGCTATTATGGCACTGGAACTCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATCCCAGAAGCTAGGATCTTGAGAATGGATGTTGACACCACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGACTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACCTCCTTAAATTTGCCAGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGAGAAGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCTATTCAATTAGCTAAGAAACAAGATTTTGAAGCCTTTTACCGTTATGAAATGGCGATTCGCCGTCAGATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGGATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-535)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.395	100	0.481