Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACE8TD_RS12155 Genome accession   NZ_CP170116
Coordinates   2565197..2566567 (-) Length   456 a.a.
NCBI ID   WP_010720638.1    Uniprot ID   A0A1V8X781
Organism   Enterococcus hirae strain 323p     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2560197..2571567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE8TD_RS12140 (ACE8TD_12140) epsC 2561477..2562013 (-) 537 WP_010720635.1 serine O-acetyltransferase EpsC -
  ACE8TD_RS12145 (ACE8TD_12145) gltX 2562326..2563786 (-) 1461 WP_010720636.1 glutamate--tRNA ligase -
  ACE8TD_RS12150 (ACE8TD_12150) - 2564003..2565163 (-) 1161 WP_034867277.1 PIN/TRAM domain-containing protein -
  ACE8TD_RS12155 (ACE8TD_12155) radA 2565197..2566567 (-) 1371 WP_010720638.1 DNA repair protein RadA Machinery gene
  ACE8TD_RS12160 (ACE8TD_12160) - 2566618..2567148 (-) 531 WP_010720639.1 dUTP diphosphatase -
  ACE8TD_RS12165 (ACE8TD_12165) proB 2567350..2568156 (+) 807 WP_010720640.1 glutamate 5-kinase -
  ACE8TD_RS12170 (ACE8TD_12170) - 2568153..2569397 (+) 1245 WP_010737261.1 glutamate-5-semialdehyde dehydrogenase -
  ACE8TD_RS12175 (ACE8TD_12175) - 2569501..2569845 (+) 345 WP_010720642.1 hypothetical protein -
  ACE8TD_RS12180 (ACE8TD_12180) - 2569934..2570251 (-) 318 WP_010737260.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49925.56 Da        Isoelectric Point: 7.3886

>NTDB_id=1055185 ACE8TD_RS12155 WP_010720638.1 2565197..2566567(-) (radA) [Enterococcus hirae strain 323p]
MAKKAKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIAEVIPKKEPRVKTKLDELN
RVLGGGVVPGSMILIGGDPGIGKSTLLLQVSQQLADIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEQISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLEGATGSAIVVAMEGTRPILVEIQALVTPTMYG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGGWNPPTGIEVVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1055185 ACE8TD_RS12155 WP_010720638.1 2565197..2566567(-) (radA) [Enterococcus hirae strain 323p]
ATGGCAAAAAAAGCGAAAGTCCAGTTTGTTTGCCAAAATTGTGGCTATGTTTCGCCTAAATTCTTAGGGCGTTGCCCAAA
TTGCGGGAAATGGAACACGATGGTCGAGGAAATCGAGCAAGATACGACTGACCGTAGAACACGAACAAGTTTGACTGGAG
AAAAAGCCAAACCAACGAAAATTGCAGAAGTGATTCCTAAAAAGGAACCACGAGTCAAAACAAAATTAGATGAATTGAAT
CGAGTTTTGGGCGGGGGCGTCGTTCCGGGGTCAATGATTTTGATTGGTGGCGATCCAGGCATCGGTAAATCCACATTACT
TTTACAAGTATCCCAACAATTAGCTGATATCGGCGGGAAAGTATTATATGTTTCTGGAGAAGAAAGTGCAGAACAAATTA
AATTACGTGCCGAGCGTTTGGGTTCAATAAATACAGAATTTTACTTATATGCTGAAACTGATATGAATGAAATTAGCCGT
GCCATCGAACAGATTTCACCAGATTATGTGATCATTGATTCGATTCAAACGATGACACAACCTGATATTACGAGTGTAGC
AGGGAGTGTCAGTCAAGTGAGAGAAACGACTGCTGAGTTGTTGAAAATAGCGAAAACAAATGGGATTGCGATTTTTATCG
TAGGACATGTAACGAAAGAAGGATCGATTGCGGGTCCACGTATGCTTGAACATATGGTTGATACTGTTTTGTATTTTGAA
GGAGAACAGCATCATAGTTTTCGTATTTTGAGAGCAGTAAAGAATCGATTTGGTTCAACAAATGAAATTGGTATATTTGA
AATGCATGAACATGGATTAGAAGAAGTTGCGAATCCTTCCCAAATCTTTTTGGAAGAACGATTAGAAGGAGCAACAGGCT
CAGCGATTGTTGTGGCGATGGAAGGTACCCGCCCAATTTTAGTGGAAATCCAAGCACTGGTAACACCAACAATGTATGGA
AATGCTAAACGAACCACTACTGGCTTAGATTTTAACCGAGTATCATTGATCATGGCAGTTCTCGAAAAACGAGCAGGCTT
ACTGTTGCAGAATCAAGATGCTTACTTAAAAGCAGCAGGTGGGGTAAAATTAAATGAACCAGCCATTGATTTAGCGATTG
CTGTGAGTATTGCTTCCAGTTACAAAGAAAAAGGGACACAACCGACAGAATGTTTTATTGGCGAAATCGGGTTGACGGGC
GAAATTCGCCGTGTCAGTCATATTGAACAGCGTGTCAAAGAAGTACAAAAGCTGGGTTTCACAAAAGTTTATTTGCCAAA
GAACAATCTAGGAGGGTGGAATCCTCCGACTGGCATTGAAGTCGTAGGTGTCGCTACCTTGGCGGAAACGTTAAAACGTG
TCTTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V8X781

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667

Multiple sequence alignment