Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACE6HX_RS11220 Genome accession   NZ_CP170098
Coordinates   2219023..2220093 (-) Length   356 a.a.
NCBI ID   WP_106039253.1    Uniprot ID   -
Organism   Bacillus pumilus strain X14-67     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2214023..2225093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE6HX_RS11170 (ACE6HX_11170) sipW 2214117..2214689 (-) 573 WP_066031752.1 signal peptidase I SipW -
  ACE6HX_RS11175 (ACE6HX_11175) tapA 2214741..2215268 (-) 528 WP_375565906.1 amyloid fiber anchoring/assembly protein TapA -
  ACE6HX_RS11180 (ACE6HX_11180) - 2215568..2215846 (+) 279 WP_375567698.1 DUF3889 domain-containing protein -
  ACE6HX_RS11185 (ACE6HX_11185) - 2215880..2216074 (-) 195 WP_024718846.1 YqzE family protein -
  ACE6HX_RS11190 (ACE6HX_11190) - 2216133..2216516 (-) 384 WP_034661288.1 competence type IV pilus minor pilin ComGG -
  ACE6HX_RS11195 (ACE6HX_11195) comGF 2216513..2217013 (-) 501 WP_034661289.1 competence type IV pilus minor pilin ComGF -
  ACE6HX_RS11200 (ACE6HX_11200) comGE 2216952..2217269 (-) 318 WP_375565908.1 competence type IV pilus minor pilin ComGE -
  ACE6HX_RS11205 (ACE6HX_11205) comGD 2217253..2217699 (-) 447 WP_066030666.1 competence type IV pilus minor pilin ComGD -
  ACE6HX_RS11210 (ACE6HX_11210) comGC 2217692..2217985 (-) 294 WP_375565911.1 competence type IV pilus major pilin ComGC Machinery gene
  ACE6HX_RS11215 (ACE6HX_11215) comGB 2218002..2219042 (-) 1041 WP_106039252.1 competence type IV pilus assembly protein ComGB -
  ACE6HX_RS11220 (ACE6HX_11220) comGA 2219023..2220093 (-) 1071 WP_106039253.1 competence type IV pilus ATPase ComGA Machinery gene
  ACE6HX_RS11225 (ACE6HX_11225) - 2220271..2221143 (-) 873 WP_106039255.1 STAS domain-containing protein -
  ACE6HX_RS11235 (ACE6HX_11235) - 2221367..2221747 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACE6HX_RS11240 (ACE6HX_11240) - 2221959..2222201 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACE6HX_RS11245 (ACE6HX_11245) - 2222250..2222888 (-) 639 WP_060596838.1 MBL fold metallo-hydrolase -
  ACE6HX_RS11250 (ACE6HX_11250) - 2223046..2223219 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  ACE6HX_RS11255 (ACE6HX_11255) - 2223244..2223558 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  ACE6HX_RS11260 (ACE6HX_11260) - 2223636..2224889 (-) 1254 WP_060596687.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40335.70 Da        Isoelectric Point: 8.8741

>NTDB_id=1055023 ACE6HX_RS11220 WP_106039253.1 2219023..2220093(-) (comGA) [Bacillus pumilus strain X14-67]
MYDIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASIRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDLIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1055023 ACE6HX_RS11220 WP_106039253.1 2219023..2220093(-) (comGA) [Bacillus pumilus strain X14-67]
TTGTATGATATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
AAAAGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGGATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCATCAGATTATTATCATTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACAAATTCAGGGAAAACAACAACCCTTTATTCATTAATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGTGCTATTTTAAGACATGATCCAGATCTGATTGTGCTAG
GAGAAATAAGAGATGCCGAAACGGCCCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACGCTTCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTTACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTAGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638

Multiple sequence alignment