Detailed information NTG1055000

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: ACE6HX_RS05140
• Genome accession: NZ_CP170098
• Coordinates: 1052342..1056046 (+)
• Length: 1234 a.a.
• NCBI ID: WP_375567198.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain X14-67
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1047342..1061046

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE6HX_RS05125 (ACE6HX_05125)	-	1047633..1048043 (+)	411	WP_034663477.1	DoxX family protein	-
ACE6HX_RS05130 (ACE6HX_05130)	-	1048241..1048687 (-)	447	WP_106039548.1	ferritin family protein	-
ACE6HX_RS05135 (ACE6HX_05135)	addB	1048855..1052364 (+)	3510	WP_066031070.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
ACE6HX_RS05140 (ACE6HX_05140)	addA	1052342..1056046 (+)	3705	WP_375567198.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
ACE6HX_RS05145 (ACE6HX_05145)	sbcD	1056073..1057248 (+)	1176	WP_060595952.1	exonuclease subunit SbcD	-
ACE6HX_RS05150 (ACE6HX_05150)	sbcC	1057245..1060640 (+)	3396	WP_375567200.1	exonuclease subunit SbcC	-
ACE6HX_RS05155 (ACE6HX_05155)	-	1060660..1060959 (+)	300	WP_060595954.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142379.47 Da
• Isoelectric Point: 5.0684

>NTDB_id=1055000 ACE6HX_RS05140 WP_375567198.1 1052342..1056046(+) (addA) [Bacillus pumilus strain X14-67]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPLPEQWMRAFLSLYDVDALTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHITLPSEEPYDESRIRHLLESLQQRDLLTDEQVQSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLFLLDYKTDRVQGRYTGGLDAAEPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=1055000 ACE6HX_RS05140 WP_375567198.1 1052342..1056046(+) (addA) [Bacillus pumilus strain X14-67]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGAAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATTACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCCCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGACCCAGGCTTTCGTTTGGCAG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTCGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGCCATGACTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCTAGGTCTCATCCTTTACCTGAGCAATGGATGAGGGCATTCCTTTCACTTTATGATGTAGATGCACTAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAGCTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GTGACGAATATGACCCTGCTTTATTAGAAAAAGCCACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTCGAGAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTCGTACAAGAAACGATTTTAAAGCTTGTGTCTAAAGGGGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGA
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTCCTTGTCCACTTAG
ATCAGCAGGAAGCGGAAACCGGCGAAGAGCGAGAAGAACTTGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCCCCGCAAATGATGGAAGAATTAAAGAAGCAGGGAATCCCAGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCGTATCAG
GATATTCCGCTTGCAGCTGTTTTAAGATCACCTATTGTTCATTTAGATGAAAATGAAATGGCACTTATTCGAACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAATTAG
AGCGATTTTTCCAATTGCTGCGTAAGTGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTATAC
CGAGATACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
TCGAGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTTCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACTGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCAGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGGGTCTTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCGACTGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGCCCTGCCCTTATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGCGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
CTAAAGGAAGATATGTCACTTGAAAAGAAGCAGCAAGACGAGGTAGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGACTTTCATGGAAATATCCATATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGGATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
CATGCAGCATATCACGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAAGGCACCTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAAGTCCAGTCAATTGATCAAGAAGGAATTGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTGAAAGCGGACTGGGTAAAACGAGAGGTATCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATTTTTACTAG
ATTATAAAACGGATAGAGTGCAAGGCAGATATACAGGTGGACTTGACGCTGCTGAACCTATCTTGAAAAAGCGCTATGAA
ACCCAAATAGCGTTATATGCAAAAGCAGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGA
TGGGAATTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.343	100	0.665