Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BCS84_RS02840 Genome accession   NZ_CP170046
Coordinates   607131..608156 (+) Length   341 a.a.
NCBI ID   WP_016767986.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.222.45.E8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 602131..613156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCS84_RS02825 (BCS84_02825) - 602482..603210 (-) 729 WP_010435300.1 glutathione peroxidase -
  BCS84_RS02830 (BCS84_02830) oxyR 603432..604325 (+) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -
  BCS84_RS02835 (BCS84_02835) - 604421..606979 (-) 2559 WP_010435294.1 penicillin-binding protein 1A -
  BCS84_RS02840 (BCS84_02840) pilM 607131..608156 (+) 1026 WP_016767986.1 type IV pilus assembly protein PilM Machinery gene
  BCS84_RS02845 (BCS84_02845) pilN 608140..608754 (+) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  BCS84_RS02850 (BCS84_02850) pilO 608747..609376 (+) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  BCS84_RS02855 (BCS84_02855) pilP 609363..609881 (+) 519 WP_010435282.1 pilus assembly protein PilP Machinery gene
  BCS84_RS02860 (BCS84_02860) pilQ 609941..611632 (+) 1692 WP_016787694.1 type IV pilus secretin PilQ Machinery gene
  BCS84_RS02865 (BCS84_02865) aroK 611809..612327 (+) 519 WP_009848907.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38112.77 Da        Isoelectric Point: 6.8348

>NTDB_id=1054913 BCS84_RS02840 WP_016767986.1 607131..608156(+) (pilM) [Vibrio cyclitrophicus strain 10N.222.45.E8]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGATSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=1054913 BCS84_RS02840 WP_016767986.1 607131..608156(+) (pilM) [Vibrio cyclitrophicus strain 10N.222.45.E8]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGAGCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.097

99.413

0.528
  pilM Vibrio campbellii strain DS40M4

47.661

100

0.478

Multiple sequence alignment