Detailed information NTG1054807

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BCU15_RS14140
• Genome accession: NZ_CP170028
• Coordinates: 3146294..3147319 (-)
• Length: 341 a.a.
• NCBI ID: WP_016787692.1
• Uniprot ID: A0AAN0LM26
• Organism: Vibrio cyclitrophicus strain 10N.261.55.B9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3141294..3152319

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BCU15_RS14115 (BCU15_14115)	aroK	3142123..3142641 (-)	519	WP_009848907.1	shikimate kinase AroK	-
BCU15_RS14120 (BCU15_14120)	pilQ	3142818..3144509 (-)	1692	WP_102341165.1	type IV pilus secretin PilQ	Machinery gene
BCU15_RS14125 (BCU15_14125)	pilP	3144569..3145087 (-)	519	WP_016787693.1	pilus assembly protein PilP	Machinery gene
BCU15_RS14130 (BCU15_14130)	pilO	3145074..3145703 (-)	630	WP_010435284.1	type 4a pilus biogenesis protein PilO	Machinery gene
BCU15_RS14135 (BCU15_14135)	pilN	3145696..3146310 (-)	615	WP_010435288.1	PilN domain-containing protein	Machinery gene
BCU15_RS14140 (BCU15_14140)	pilM	3146294..3147319 (-)	1026	WP_016787692.1	type IV pilus assembly protein PilM	Machinery gene
BCU15_RS14145 (BCU15_14145)	-	3147471..3150029 (+)	2559	WP_198591911.1	penicillin-binding protein 1A	-
BCU15_RS14150 (BCU15_14150)	oxyR	3150125..3151018 (-)	894	WP_010435297.1	DNA-binding transcriptional regulator OxyR	-
BCU15_RS14155 (BCU15_14155)	-	3151240..3151968 (+)	729	WP_010435300.1	glutathione peroxidase	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38128.77 Da
• Isoelectric Point: 6.8348

>NTDB_id=1054807 BCU15_RS14140 WP_016787692.1 3146294..3147319(-) (pilM) [Vibrio cyclitrophicus strain 10N.261.55.B9]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=1054807 BCU15_RS14140 WP_016787692.1 3146294..3147319(-) (pilM) [Vibrio cyclitrophicus strain 10N.261.55.B9]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGATCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	53.392	99.413	0.531
pilM	Vibrio campbellii strain DS40M4	47.661	100	0.478