Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   R8588_RS05710 Genome accession   NZ_AP028401
Coordinates   1087604..1088563 (+) Length   319 a.a.
NCBI ID   WP_002869618.1    Uniprot ID   -
Organism   Campylobacter jejuni strain BCH-11527     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1066253..1097900 1087604..1088563 within 0


Gene organization within MGE regions


Location: 1066253..1097900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8588_RS05595 (B11527_10990) pglI 1066253..1067182 (-) 930 WP_002860218.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  R8588_RS05600 (B11527_11000) pglH 1067175..1068254 (-) 1080 WP_079268903.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  R8588_RS05605 (B11527_11010) pglK 1068251..1069945 (-) 1695 WP_052789827.1 ABC-type lipopolysaccharide transporter PglK -
  R8588_RS05610 (B11527_11020) galE 1069939..1070925 (-) 987 WP_002874249.1 UDP-glucose 4-epimerase GalE -
  R8588_RS05615 (B11527_11030) - 1070984..1071778 (-) 795 WP_020247039.1 3'-5' exonuclease -
  R8588_RS05620 (B11527_11040) waaC 1071845..1072873 (+) 1029 WP_020247040.1 lipopolysaccharide heptosyltransferase I -
  R8588_RS05625 (B11527_11050) htrB 1072863..1073741 (+) 879 WP_052789826.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  R8588_RS05630 (B11527_11060) - 1073738..1074439 (+) 702 WP_002869594.1 glycosyltransferase family 2 protein -
  R8588_RS05635 (B11527_11070) rfbA 1074450..1075328 (+) 879 WP_002869595.1 glucose-1-phosphate thymidylyltransferase RfbA -
  R8588_RS05640 (B11527_11080) rfbB 1075328..1076359 (+) 1032 WP_002860226.1 dTDP-glucose 4,6-dehydratase -
  R8588_RS05645 (B11527_11090) wlaRA 1076362..1076811 (+) 450 WP_002912325.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRA -
  R8588_RS05650 (B11527_11100) wlaRB 1076808..1077212 (+) 405 WP_002860228.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRB -
  R8588_RS05655 (B11527_11110) - 1077202..1077643 (+) 442 Protein_1092 DapH/DapD/GlmU-related protein -
  R8588_RS05660 (B11527_11120) - 1077665..1078192 (+) 528 WP_002869598.1 GNAT family N-acetyltransferase -
  R8588_RS08715 - 1078213..1078470 (+) 258 Protein_1094 hypothetical protein -
  R8588_RS05665 (B11527_11130) - 1078489..1079553 (+) 1065 WP_002869599.1 glycosyltransferase family 2 protein -
  R8588_RS05670 (B11527_11140) - 1079566..1080618 (+) 1053 WP_002869601.1 glycosyltransferase family 2 protein -
  R8588_RS05675 (B11527_11150) wlaRG 1080584..1081669 (+) 1086 WP_025998025.1 class E lipooligosaccharide biosynthesis aminotransferase WlaRG -
  R8588_RS05680 (B11527_11160) - 1081696..1082955 (-) 1260 WP_002869603.1 DUF2972 domain-containing protein -
  R8588_RS05685 - 1082952..1083975 (-) 1024 Protein_1099 hypothetical protein -
  R8588_RS05690 (B11527_11200) - 1084046..1084819 (+) 774 WP_002869611.1 glycosyltransferase family 25 protein -
  R8588_RS05695 (B11527_11210) wlaTB 1084812..1085768 (+) 957 WP_002869613.1 class E lipooligosaccharide biosynthesis protein WlaTB -
  R8588_RS05700 (B11527_11220) yibB 1085765..1086724 (+) 960 WP_002869615.1 protein YibB -
  R8588_RS05705 (B11527_11230) waaV 1086727..1087545 (-) 819 WP_002869617.1 glycosyltransferase family 2 protein -
  R8588_RS05710 (B11527_11240) waaF 1087604..1088563 (+) 960 WP_002869618.1 lipopolysaccharide heptosyltransferase II Regulator
  R8588_RS05715 (B11527_11250) gmhA 1088544..1089104 (-) 561 WP_002869619.1 D-sedoheptulose 7-phosphate isomerase -
  R8588_RS05720 (B11527_11260) rfaE1 1089101..1090486 (-) 1386 WP_002867399.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  R8588_RS05725 (B11527_11270) rfaD 1090479..1091432 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  R8588_RS05730 (B11527_11280) gmhB 1091433..1091994 (-) 562 Protein_1108 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  R8588_RS05735 (B11527_11290) - 1092075..1092377 (+) 303 WP_002852762.1 c-type cytochrome -
  R8588_RS05740 (B11527_11300) ccoS 1092400..1092606 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  R8588_RS05745 (B11527_11310) - 1092603..1094960 (-) 2358 WP_079268904.1 heavy metal translocating P-type ATPase -
  R8588_RS05750 (B11527_11320) rho 1095069..1096367 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  R8588_RS05755 (B11527_11330) dnaX 1096371..1097900 (+) 1530 WP_044258566.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36850.61 Da        Isoelectric Point: 10.3923

>NTDB_id=105470 R8588_RS05710 WP_002869618.1 1087604..1088563(+) (waaF) [Campylobacter jejuni strain BCH-11527]
MKIFIHLPTWLGDAVMASPALYVIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNRKKILGLNPGA
SFGSAKRWDASYFAKVALNFNQSHEILIFGAGKVEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNQNAKLVHLDLACMPCMQKICPLKHHKCMKDLKPQRVIEEAKNLLKNPHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=105470 R8588_RS05710 WP_002869618.1 1087604..1088563(+) (waaF) [Campylobacter jejuni strain BCH-11527]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGTTATAAAAGAACA
TTTTAAAAATGCTCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATCGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTAGCTTTAAATTTCAACCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGTCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATTGCTTTTTGTGATCTTTTCATAACAAATGACAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATCAAAACGCAAAATTAGTACACTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAATCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTCAAAGGGTTATAGAAGAAGCTAAAAATTTACTTAAAAACCCTCATCTATAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

94.357

100

0.944

Multiple sequence alignment