Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   BCU67_RS02960 Genome accession   NZ_CP170019
Coordinates   622771..623385 (+) Length   204 a.a.
NCBI ID   WP_010435288.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.49.B11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 617771..628385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU67_RS02945 (BCU67_02945) oxyR 618063..618956 (+) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -
  BCU67_RS02950 (BCU67_02950) - 619052..621610 (-) 2559 WP_010435294.1 penicillin-binding protein 1A -
  BCU67_RS02955 (BCU67_02955) pilM 621762..622787 (+) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene
  BCU67_RS02960 (BCU67_02960) pilN 622771..623385 (+) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  BCU67_RS02965 (BCU67_02965) pilO 623378..624007 (+) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  BCU67_RS02970 (BCU67_02970) pilP 623994..624512 (+) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  BCU67_RS02975 (BCU67_02975) pilQ 624572..626263 (+) 1692 WP_016787694.1 type IV pilus secretin PilQ Machinery gene
  BCU67_RS02980 (BCU67_02980) aroK 626440..626958 (+) 519 WP_009848907.1 shikimate kinase AroK -
  BCU67_RS02985 (BCU67_02985) aroB 626983..628071 (+) 1089 WP_010435273.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23345.82 Da        Isoelectric Point: 9.3072

>NTDB_id=1054639 BCU67_RS02960 WP_010435288.1 622771..623385(+) (pilN) [Vibrio cyclitrophicus strain 10N.286.49.B11]
MLHQINLLSWRDEIRAQHKKRFIHLVILGVIIALAGQWAVGHYFHDQQAKQQARLSYLDKYIAVLDRQIQSLKVAEQEHK
AILTRLAVVESLQFGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKEVEMHSIV
HNRKRFNKEFQSFKVSFVFSPVSLENTTAEGTTANIKEKGANHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1054639 BCU67_RS02960 WP_010435288.1 622771..623385(+) (pilN) [Vibrio cyclitrophicus strain 10N.286.49.B11]
ATGTTGCATCAAATTAACCTGTTGTCGTGGCGTGATGAGATTCGCGCTCAGCATAAAAAGCGTTTCATTCATCTGGTCAT
TCTTGGCGTCATCATTGCGCTTGCCGGGCAATGGGCGGTGGGACACTATTTTCATGACCAACAAGCCAAGCAGCAAGCGC
GCCTCAGTTATCTCGATAAGTACATCGCAGTGCTCGACCGACAAATCCAGTCATTAAAAGTCGCAGAGCAAGAGCACAAA
GCCATCCTTACACGACTTGCTGTCGTGGAGTCTTTACAGTTCGGGCGCAATAAAACCACCGACTTTATGAACCTGATGCC
GGAGCTGATTCCTGAGGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGACA
GCACCGCTCGTCTAGCCACCATGTTGGACAACCTAGAACGTTCTGACTCTCTTAAAGAAGTTGAGATGCACTCTATTGTT
CATAACCGTAAGCGCTTCAATAAAGAGTTTCAGAGCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTTTCTTTAGAAAACAC
AACTGCAGAGGGCACAACTGCGAACATAAAAGAGAAGGGGGCGAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

63.731

94.608

0.603
  pilN Vibrio campbellii strain DS40M4

61.78

93.627

0.578

Multiple sequence alignment