Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   U2B25_RS08395 Genome accession   NZ_CP169947
Coordinates   1712102..1713076 (-) Length   324 a.a.
NCBI ID   WP_000697221.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BSN57-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1707102..1718076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2B25_RS08355 (U2B25_08355) gcvT 1707603..1708694 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  U2B25_RS08360 (U2B25_08360) - 1708853..1709377 (-) 525 WP_001015120.1 shikimate kinase -
  U2B25_RS08365 (U2B25_08365) - 1709367..1709513 (-) 147 WP_001792109.1 hypothetical protein -
  U2B25_RS08370 (U2B25_08370) comGF 1709610..1710107 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  U2B25_RS08375 (U2B25_08375) comGE 1710025..1710324 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  U2B25_RS08380 (U2B25_08380) comGD 1710311..1710757 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  U2B25_RS08385 (U2B25_08385) comGC 1710735..1711046 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  U2B25_RS08390 (U2B25_08390) comGB 1711060..1712130 (-) 1071 WP_064265422.1 competence type IV pilus assembly protein ComGB Machinery gene
  U2B25_RS08395 (U2B25_08395) comGA 1712102..1713076 (-) 975 WP_000697221.1 competence type IV pilus ATPase ComGA Machinery gene
  U2B25_RS08400 (U2B25_08400) - 1713128..1713751 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  U2B25_RS08405 (U2B25_08405) - 1713748..1714077 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  U2B25_RS08410 (U2B25_08410) - 1714077..1715063 (-) 987 WP_000161311.1 ROK family glucokinase -
  U2B25_RS08415 (U2B25_08415) - 1715060..1715263 (-) 204 WP_000087561.1 YqgQ family protein -
  U2B25_RS08420 (U2B25_08420) - 1715244..1716707 (-) 1464 WP_001017852.1 rhomboid family protein -
  U2B25_RS08425 (U2B25_08425) - 1716719..1717258 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  U2B25_RS08430 (U2B25_08430) rpmG 1717467..1717616 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36925.97 Da        Isoelectric Point: 8.8537

>NTDB_id=1054101 U2B25_RS08395 WP_000697221.1 1712102..1713076(-) (comGA) [Staphylococcus aureus strain BSN57-2]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTRAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1054101 U2B25_RS08395 WP_000697221.1 1712102..1713076(-) (comGA) [Staphylococcus aureus strain BSN57-2]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAGAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997

Multiple sequence alignment