Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   U2B25_RS00185 Genome accession   NZ_CP169947
Coordinates   41283..42644 (-) Length   453 a.a.
NCBI ID   WP_001631036.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BSN57-2     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 35051..88435 41283..42644 within 0
SCCmec 39658..104329 41283..42644 within 0


Gene organization within MGE regions


Location: 35051..104329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2B25_RS00145 (U2B25_00145) - 35594..35854 (-) 261 Protein_26 IS5/IS1182 family transposase -
  U2B25_RS00150 (U2B25_00150) - 35913..36410 (-) 498 WP_001631030.1 GNAT family N-acetyltransferase -
  U2B25_RS00155 (U2B25_00155) - 37082..37492 (-) 411 WP_001631031.1 DUF2871 domain-containing protein -
  U2B25_RS00160 (U2B25_00160) - 37655..37875 (-) 221 Protein_29 hypothetical protein -
  U2B25_RS00165 (U2B25_00165) - 37890..38393 (-) 504 WP_001631032.1 DUF1643 domain-containing protein -
  U2B25_RS00170 (U2B25_00170) - 38409..38720 (-) 312 WP_001631033.1 DUF960 domain-containing protein -
  U2B25_RS00175 (U2B25_00175) - 38807..39157 (-) 351 WP_001631034.1 SAUGI family uracil-DNA glycosylase inhibitor -
  U2B25_RS00180 (U2B25_00180) ccrB 39658..41286 (-) 1629 WP_001631035.1 cassette chromosome recombinase CcrB Machinery gene
  U2B25_RS00185 (U2B25_00185) ccrA 41283..42644 (-) 1362 WP_001631036.1 cassette chromosome recombinase CcrA Machinery gene
  U2B25_RS00190 (U2B25_00190) - 42831..43460 (-) 630 Protein_35 hypothetical protein -
  U2B25_RS00195 (U2B25_00195) - 43469..43807 (+) 339 Protein_36 recombinase family protein -
  U2B25_RS00200 (U2B25_00200) - 43919..44278 (-) 360 WP_196506795.1 hypothetical protein -
  U2B25_RS00205 (U2B25_00205) - 44485..44811 (+) 327 WP_000391495.1 ArsR/SmtB family transcription factor -
  U2B25_RS00210 (U2B25_00210) - 45141..47066 (+) 1926 WP_320234022.1 heavy metal translocating P-type ATPase -
  U2B25_RS00215 (U2B25_00215) - 48161..48790 (+) 630 WP_001053439.1 hypothetical protein -
  U2B25_RS00220 (U2B25_00220) - 48805..49047 (+) 243 WP_000931963.1 hypothetical protein -
  U2B25_RS00225 (U2B25_00225) - 49446..50297 (+) 852 WP_000832687.1 IS3 family transposase -
  U2B25_RS00230 (U2B25_00230) arcC 50397..51326 (-) 930 WP_000036756.1 carbamate kinase -
  U2B25_RS00235 (U2B25_00235) argF 51346..52344 (-) 999 WP_000793596.1 ornithine carbamoyltransferase -
  U2B25_RS00240 (U2B25_00240) - 52382..53071 (-) 690 WP_000272781.1 Crp/Fnr family transcriptional regulator -
  U2B25_RS00245 (U2B25_00245) arcD 53113..54534 (-) 1422 WP_000344142.1 arginine-ornithine antiporter -
  U2B25_RS00250 (U2B25_00250) arcA 54620..55855 (-) 1236 WP_000249804.1 arginine deiminase -
  U2B25_RS00255 (U2B25_00255) - 56124..56570 (-) 447 WP_000711815.1 arginine repressor -
  U2B25_RS00260 (U2B25_00260) - 57143..57825 (+) 683 Protein_49 type I restriction enzyme endonuclease domain-containing protein -
  U2B25_RS00265 (U2B25_00265) - 58096..58527 (-) 432 WP_000475804.1 universal stress protein -
  U2B25_RS00270 (U2B25_00270) - 59409..59735 (-) 327 WP_000916431.1 hypothetical protein -
  U2B25_RS00275 (U2B25_00275) - 59751..60425 (+) 675 WP_057504635.1 IS6 family transposase -
  U2B25_RS00280 (U2B25_00280) - 61185..61418 (-) 234 WP_000400093.1 hypothetical protein -
  U2B25_RS00285 (U2B25_00285) - 61664..66856 (+) 5193 WP_411197834.1 E domain-containing protein -
  U2B25_RS00290 (U2B25_00290) - 66980..68596 (+) 1617 WP_000393941.1 hypothetical protein -
  U2B25_RS00295 (U2B25_00295) - 68756..70267 (-) 1512 WP_000640645.1 glycosyltransferase family 4 protein -
  U2B25_RS00300 (U2B25_00300) - 70290..71948 (-) 1659 WP_001621813.1 Mur ligase family protein -
  U2B25_RS00305 (U2B25_00305) - 71942..72340 (-) 399 WP_001621810.1 hypothetical protein -
  U2B25_RS00310 (U2B25_00310) - 73422..74678 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  U2B25_RS00315 (U2B25_00315) - 75093..75332 (+) 240 WP_001282728.1 hypothetical protein -
  U2B25_RS00320 (U2B25_00320) - 75364..76038 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  U2B25_RS00325 (U2B25_00325) - 76129..77112 (-) 984 Protein_62 protein rep -
  U2B25_RS00330 (U2B25_00330) mobV 77357..78619 (-) 1263 WP_000119405.1 MobV family relaxase -
  U2B25_RS00335 (U2B25_00335) bleO 79127..79531 (-) 405 WP_001242578.1 bleomycin binding protein -
  U2B25_RS00340 (U2B25_00340) aadD1 79748..80518 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  U2B25_RS00345 (U2B25_00345) - 80711..81385 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  U2B25_RS00350 (U2B25_00350) - 81650..82393 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  U2B25_RS00355 (U2B25_00355) - 82490..82918 (+) 429 WP_000872606.1 MaoC family dehydratase -
  U2B25_RS00360 (U2B25_00360) mecA 82964..84973 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  U2B25_RS00365 (U2B25_00365) mecR1 85070..86827 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  U2B25_RS00370 (U2B25_00370) mecI 86827..87198 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  U2B25_RS00375 (U2B25_00375) psm-mec 87283..87351 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  U2B25_RS00380 (U2B25_00380) - 87685..88815 (+) 1131 WP_010922808.1 ROK family transcriptional regulator -
  U2B25_RS00385 (U2B25_00385) cstB 88929..90263 (-) 1335 Protein_74 persulfide dioxygenase-sulfurtransferase CstB -
  U2B25_RS00390 (U2B25_00390) - 90296..91360 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  U2B25_RS00395 (U2B25_00395) cstR 91496..91756 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  U2B25_RS00400 (U2B25_00400) - 91756..92394 (+) 639 Protein_77 sulfite exporter TauE/SafE family protein -
  U2B25_RS00405 (U2B25_00405) - 92739..93401 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  U2B25_RS00410 (U2B25_00410) - 93793..93852 (+) 60 WP_010959190.1 erythromycin resistance leader peptide -
  U2B25_RS00415 (U2B25_00415) erm(A) 93935..94666 (+) 732 WP_320234976.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  U2B25_RS00420 (U2B25_00420) - 94792..95574 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  U2B25_RS00425 (U2B25_00425) - 95725..96102 (-) 378 WP_000361059.1 DUF6262 family protein -
  U2B25_RS00430 (U2B25_00430) - 96109..98001 (-) 1893 WP_001557544.1 site-specific integrase -
  U2B25_RS00435 (U2B25_00435) - 97998..99083 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  U2B25_RS00440 (U2B25_00440) - 99202..99519 (-) 318 WP_000659050.1 JAB domain-containing protein -
  U2B25_RS00445 (U2B25_00445) - 99540..100046 (-) 507 WP_001092169.1 DUF1643 domain-containing protein -
  U2B25_RS00450 (U2B25_00450) - 100064..100375 (-) 312 WP_000700853.1 DUF960 family protein -
  U2B25_RS00455 (U2B25_00455) - 100462..100812 (-) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  U2B25_RS00460 (U2B25_00460) ccrB 101330..102958 (-) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  U2B25_RS00465 (U2B25_00465) ccrA 102980..104329 (-) 1350 WP_196581173.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 53316.45 Da        Isoelectric Point: 9.8596

>NTDB_id=1054079 U2B25_RS00185 WP_001631036.1 41283..42644(-) (ccrA) [Staphylococcus aureus strain BSN57-2]
MEEAIAYVRQSTLKQQSLATQKSLIMDTAKQYGWSNVAFYDDKKTGRHTKRSGYQKMVEIITSGKCKVLCCYRLNRLHRN
LKNAIQFFEICKKHHITIISVNDGYFDLSKEFDCFRLNILMSLAEMESNNISEQTKNGIREKAKQGKLITTHAPFGYRYR
KGCFVVQEEEAYTVKSVYRWYLQGLGYKKISQHLDSNPNLIPRKPYQVRNILLNPNYCGRVINKYGTFNDIVPPIIDIDT
FEEAQERRIHKQLNRSNSRNKLKRRIKCPYCQSTLTNLTIKKENYSLRYYVCPKNMNDAYRTCPFKSINAPELESEVLDT
CKKYFEEQSFHKRLNNTILKVLKQQQMKHKETHLTQEQLIEKLAQNRIDVKTFKRLSASCKSEENYSTYSTYQIDDKLKY
VIKNKITLQDIAPFIDCIKITQTKQLQGIYFKNSPLNIVEQSHLITPERNEVI

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1054079 U2B25_RS00185 WP_001631036.1 41283..42644(-) (ccrA) [Staphylococcus aureus strain BSN57-2]
ATGGAAGAAGCGATTGCTTATGTACGTCAAAGTACACTCAAACAACAAAGCTTAGCTACTCAAAAATCGCTCATTATGGA
TACCGCTAAGCAATATGGATGGTCTAATGTGGCGTTTTATGATGATAAGAAAACAGGTCGACATACAAAACGTTCAGGTT
ATCAAAAAATGGTAGAAATAATTACGTCTGGAAAATGTAAAGTATTGTGTTGCTATCGCTTGAACCGACTACATCGTAAT
TTGAAAAATGCCATACAATTCTTTGAAATTTGTAAAAAGCATCACATCACAATCATCAGTGTTAACGATGGATATTTTGA
TTTGTCTAAAGAGTTTGATTGCTTTCGTTTGAACATACTTATGAGTCTAGCGGAAATGGAATCAAACAATATCAGCGAAC
AAACAAAAAATGGGATTAGAGAAAAAGCCAAACAAGGTAAGCTAATCACAACTCATGCTCCGTTTGGCTATCGTTATAGA
AAAGGTTGTTTTGTTGTACAAGAAGAAGAAGCGTATACAGTTAAATCGGTTTATCGCTGGTACTTACAAGGTCTAGGCTA
CAAAAAGATTTCCCAACATTTGGATAGTAATCCTAACCTTATTCCTCGTAAGCCCTATCAAGTTAGAAATATTCTCTTGA
ACCCTAACTATTGCGGTCGTGTCATCAATAAATATGGCACGTTTAATGATATCGTTCCACCTATTATTGATATTGATACA
TTTGAAGAAGCACAAGAGCGTCGTATTCATAAACAACTCAATCGATCCAATTCTAGAAATAAGCTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAGAACTATTCTCTACGTTACTATGTTTGTCCTA
AAAATATGAATGACGCTTATCGTACATGCCCATTTAAAAGTATCAATGCACCAGAACTTGAAAGTGAAGTGCTCGACACT
TGTAAAAAGTATTTTGAAGAACAATCGTTTCATAAACGATTGAATAACACAATACTCAAAGTCCTAAAACAGCAACAAAT
GAAGCATAAAGAAACCCATCTAACACAAGAACAACTTATCGAAAAGCTAGCTCAAAATCGTATTGATGTAAAAACGTTCA
AACGTTTATCTGCTAGTTGTAAAAGTGAAGAGAATTACTCTACTTATTCAACCTACCAAATCGATGACAAATTAAAATAT
GTCATAAAAAATAAAATAACTTTACAGGATATAGCACCTTTTATCGATTGCATCAAAATAACTCAAACTAAACAATTACA
AGGAATCTACTTTAAAAACAGTCCACTCAATATCGTTGAACAATCACATTTAATAACTCCTGAAAGGAATGAAGTAATAT
GA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(168-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

54.628

97.792

0.534
  ccrA Staphylococcus aureus N315

50.564

97.792

0.494

Multiple sequence alignment