Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABUS10_RS15565 Genome accession   NZ_CP169869
Coordinates   3328857..3329657 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain Up280     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3323857..3334657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABUS10_RS15545 - 3324922..3325620 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  ABUS10_RS15550 hisS 3325649..3326941 (-) 1293 WP_000095251.1 histidine--tRNA ligase -
  ABUS10_RS15555 ispG 3326938..3328053 (-) 1116 WP_002067329.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABUS10_RS15560 - 3328075..3328866 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  ABUS10_RS15565 pilF 3328857..3329657 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABUS10_RS15570 rlmN 3329673..3330905 (-) 1233 WP_002067279.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABUS10_RS15575 ndk 3331028..3331459 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  ABUS10_RS15580 iscX 3331573..3331773 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  ABUS10_RS15585 - 3331873..3332424 (-) 552 WP_000669563.1 phosphatase PAP2 family protein -
  ABUS10_RS15590 - 3332449..3333747 (-) 1299 WP_086238243.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=1053870 ABUS10_RS15565 WP_004738086.1 3328857..3329657(-) (pilF) [Acinetobacter baumannii strain Up280]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1053870 ABUS10_RS15565 WP_004738086.1 3328857..3329657(-) (pilF) [Acinetobacter baumannii strain Up280]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment