Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABUS27_RS15125 Genome accession   NZ_CP169811
Coordinates   3177372..3178172 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain Lv371     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3172372..3183172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABUS27_RS15105 - 3173437..3174135 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  ABUS27_RS15110 hisS 3174164..3175456 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  ABUS27_RS15115 ispG 3175453..3176568 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABUS27_RS15120 - 3176590..3177381 (-) 792 WP_000405161.1 RodZ domain-containing protein -
  ABUS27_RS15125 pilF 3177372..3178172 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABUS27_RS15130 rlmN 3178188..3179420 (-) 1233 WP_000093081.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABUS27_RS15135 ndk 3179543..3179974 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  ABUS27_RS15140 iscX 3180088..3180288 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  ABUS27_RS15145 - 3180388..3180939 (-) 552 WP_000769125.1 phosphatase PAP2 family protein -
  ABUS27_RS15150 - 3180964..3182262 (-) 1299 WP_085940557.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=1053350 ABUS27_RS15125 WP_004738086.1 3177372..3178172(-) (pilF) [Acinetobacter baumannii strain Lv371]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1053350 ABUS27_RS15125 WP_004738086.1 3177372..3178172(-) (pilF) [Acinetobacter baumannii strain Lv371]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment