Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   R8922_RS06225 Genome accession   NZ_AP028390
Coordinates   1200973..1201926 (+) Length   317 a.a.
NCBI ID   WP_107701923.1    Uniprot ID   -
Organism   Campylobacter jejuni strain BCH-11411     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1182360..1212052 1200973..1201926 within 0


Gene organization within MGE regions


Location: 1182360..1212052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8922_RS06130 (B11411_12240) pglI 1182360..1183289 (-) 930 WP_002882940.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  R8922_RS06135 (B11411_12250) pglH 1183282..1184361 (-) 1080 WP_079460667.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  R8922_RS06140 (B11411_12260) pglK 1184358..1186052 (-) 1695 WP_002883263.1 ABC-type lipopolysaccharide transporter PglK -
  R8922_RS06145 (B11411_12270) galE 1186046..1187032 (-) 987 WP_306197866.1 UDP-glucose 4-epimerase GalE -
  R8922_RS06150 (B11411_12280) - 1187090..1187884 (-) 795 WP_052857795.1 3'-5' exonuclease -
  R8922_RS06155 (B11411_12290) waaC 1187950..1188978 (+) 1029 WP_052802060.1 lipopolysaccharide heptosyltransferase I -
  R8922_RS06160 (B11411_12300) htrB 1188968..1189855 (+) 888 WP_306197860.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  R8922_RS06165 (B11411_12310) - 1189852..1191399 (+) 1548 WP_306181089.1 glycosyltransferase family 2 protein -
  R8922_RS06170 (B11411_12330) - 1191403..1192574 (+) 1172 Protein_1197 glycosyltransferase family 2 protein -
  R8922_RS06175 (B11411_12340) - 1192618..1193679 (+) 1062 WP_002882966.1 beta-1,4-N-acetylgalactosaminyltransferase -
  R8922_RS06180 - 1193651..1194543 (-) 893 Protein_1199 glycosyltransferase family 2 protein -
  R8922_RS06185 - 1194632..1195511 (+) 880 Protein_1200 alpha-2,3-sialyltransferase -
  R8922_RS06190 (B11411_12390) neuB 1195512..1196552 (+) 1041 WP_107701922.1 N-acetylneuraminate synthase -
  R8922_RS06195 (B11411_12400) neuC 1196549..1197673 (+) 1125 WP_025399082.1 UDP-N-acetylglucosamine 2-epimerase -
  R8922_RS06200 (B11411_12410) - 1197666..1198283 (+) 618 WP_016818193.1 glycosyltransferase -
  R8922_RS06205 (B11411_12420) - 1198280..1198612 (+) 333 WP_019260376.1 hypothetical protein -
  R8922_RS06210 (B11411_12430) - 1198609..1199274 (+) 666 WP_002869017.1 cytidylyltransferase domain-containing protein -
  R8922_RS06215 (B11411_12440) - 1199262..1200095 (+) 834 WP_002882959.1 CatB-related O-acetyltransferase -
  R8922_RS06220 (B11411_12450) - 1200087..1200914 (-) 828 WP_052785832.1 glycosyltransferase family 2 protein -
  R8922_RS06225 (B11411_12460) waaF 1200973..1201926 (+) 954 WP_107701923.1 glycosyltransferase family 9 protein Regulator
  R8922_RS06230 (B11411_12470) - 1201933..1202688 (-) 756 WP_002869013.1 glycosyltransferase family 25 protein -
  R8922_RS06235 (B11411_12480) gmhA 1202688..1203248 (-) 561 WP_107701924.1 D-sedoheptulose 7-phosphate isomerase -
  R8922_RS06240 (B11411_12490) rfaE1 1203245..1204630 (-) 1386 WP_107701925.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  R8922_RS06245 (B11411_12500) rfaD 1204623..1205576 (-) 954 WP_052802041.1 ADP-glyceromanno-heptose 6-epimerase -
  R8922_RS06250 (B11411_12510) gmhB 1205578..1206139 (-) 562 Protein_1213 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  R8922_RS06255 (B11411_12520) - 1206220..1206522 (+) 303 WP_052785829.1 c-type cytochrome -
  R8922_RS06260 (B11411_12530) ccoS 1206546..1206752 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  R8922_RS06265 (B11411_12540) - 1206749..1209106 (-) 2358 WP_107701927.1 heavy metal translocating P-type ATPase -
  R8922_RS06270 (B11411_12550) rho 1209215..1210513 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  R8922_RS06275 (B11411_12560) dnaX 1210517..1212052 (+) 1536 WP_107701928.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 36534.16 Da        Isoelectric Point: 10.2875

>NTDB_id=105333 R8922_RS06225 WP_107701923.1 1200973..1201926(+) (waaF) [Campylobacter jejuni strain BCH-11411]
MKIFIHLPTWLGDAVMASLALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQSPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENARWVHLNLACMPCMQKTCPLKHHKCMKDLKPEIIIEVSKQLLFLN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=105333 R8922_RS06225 WP_107701923.1 1200973..1201926(+) (waaF) [Campylobacter jejuni strain BCH-11411]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCGCTTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTACAAACAAGCCCTATCCTTACGCAAAGAACTTGGCAAAATCGATTTTAGCTTTGCT
TTTAGGTCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAGCAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAGATGGGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAACCAGAAATCATCATAGAAGTAAGTAAACAATTGCTATTTCTAAATTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.675

99.054

0.918

Multiple sequence alignment