Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACE1EH_RS08620 Genome accession   NZ_CP169535
Coordinates   1778465..1778899 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain HJ-16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1773465..1783899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1EH_RS08615 (ACE1EH_08615) - 1776965..1778176 (-) 1212 WP_044801505.1 cytochrome P450 -
  ACE1EH_RS08620 (ACE1EH_08620) nucA/comI 1778465..1778899 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACE1EH_RS08625 (ACE1EH_08625) - 1779018..1779713 (+) 696 WP_015388207.1 YoaK family protein -
  ACE1EH_RS08630 (ACE1EH_08630) - 1779747..1780109 (-) 363 WP_007410383.1 hypothetical protein -
  ACE1EH_RS08635 (ACE1EH_08635) - 1780303..1781631 (-) 1329 WP_375005399.1 S8 family peptidase -
  ACE1EH_RS08640 (ACE1EH_08640) - 1781810..1782043 (+) 234 WP_285979456.1 hypothetical protein -
  ACE1EH_RS08645 (ACE1EH_08645) - 1782299..1783006 (+) 708 WP_174737268.1 poly-gamma-glutamate hydrolase family protein -
  ACE1EH_RS08650 (ACE1EH_08650) - 1783067..1783519 (+) 453 WP_375005400.1 OsmC family protein -
  ACE1EH_RS08655 (ACE1EH_08655) - 1783533..1783886 (-) 354 WP_375005401.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=1051866 ACE1EH_RS08620 WP_007611576.1 1778465..1778899(+) (nucA/comI) [Bacillus velezensis strain HJ-16]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1051866 ACE1EH_RS08620 WP_007611576.1 1778465..1778899(+) (nucA/comI) [Bacillus velezensis strain HJ-16]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATTAAAGACGCCATTTCAGCGGGTCATTCTGAGATTTGTACAATTGATAGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment