Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACE1EH_RS00585 Genome accession   NZ_CP169535
Coordinates   106651..108030 (+) Length   459 a.a.
NCBI ID   WP_375004824.1    Uniprot ID   -
Organism   Bacillus velezensis strain HJ-16     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101651..113030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1EH_RS00565 (ACE1EH_00565) ctsR 101999..102463 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  ACE1EH_RS00570 (ACE1EH_00570) - 102477..103034 (+) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  ACE1EH_RS00575 (ACE1EH_00575) - 103034..104125 (+) 1092 WP_003156398.1 protein arginine kinase -
  ACE1EH_RS00580 (ACE1EH_00580) clpC 104122..106557 (+) 2436 WP_375004823.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  ACE1EH_RS00585 (ACE1EH_00585) radA 106651..108030 (+) 1380 WP_375004824.1 DNA repair protein RadA Machinery gene
  ACE1EH_RS00590 (ACE1EH_00590) disA 108034..109116 (+) 1083 WP_007615225.1 DNA integrity scanning diadenylate cyclase DisA -
  ACE1EH_RS00595 (ACE1EH_00595) - 109230..110330 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  ACE1EH_RS00600 (ACE1EH_00600) ispD 110343..111041 (+) 699 WP_375004826.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACE1EH_RS00605 (ACE1EH_00605) ispF 111034..111510 (+) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49508.88 Da        Isoelectric Point: 7.1316

>NTDB_id=1051834 ACE1EH_RS00585 WP_375004824.1 106651..108030(+) (radA) [Bacillus velezensis strain HJ-16]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQTLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1051834 ACE1EH_RS00585 WP_375004824.1 106651..108030(+) (radA) [Bacillus velezensis strain HJ-16]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAGTGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCAAACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTGATCGGGGGCGATCCCGGCATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTAAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAACGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTGTACCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGGCCGATCCTGGTTGAAATCCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTGTCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATTATACC
TGAGGCGAATGCAGATGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(71-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627

Multiple sequence alignment