Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   R8616_RS03205 Genome accession   NZ_AP028382
Coordinates   644411..645355 (-) Length   314 a.a.
NCBI ID   WP_002844797.1    Uniprot ID   -
Organism   Campylobacter coli strain BCH-11051     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 634278..680252 644411..645355 within 0


Gene organization within MGE regions


Location: 634278..680252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8616_RS03155 (B11051_06240) - 634278..635834 (-) 1557 WP_002787752.1 DNA polymerase III subunit gamma/tau -
  R8616_RS03160 (B11051_06250) rho 635837..637135 (-) 1299 WP_002780613.1 transcription termination factor Rho -
  R8616_RS03165 (B11051_06260) - 637248..639611 (+) 2364 WP_072213620.1 heavy metal translocating P-type ATPase -
  R8616_RS03170 (B11051_06270) - 639604..639804 (+) 201 WP_002777240.1 cbb3-type cytochrome oxidase assembly protein -
  R8616_RS03175 (B11051_06280) - 639834..640133 (-) 300 WP_002777238.1 cytochrome c -
  R8616_RS03180 (B11051_06290) - 640210..640773 (+) 564 WP_002777235.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  R8616_RS03185 (B11051_06300) rfaD 640770..641723 (+) 954 WP_002796912.1 ADP-glyceromanno-heptose 6-epimerase -
  R8616_RS03190 (B11051_06310) rfaE1 641716..643101 (+) 1386 WP_002844795.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  R8616_RS03195 (B11051_06320) gmhA 643098..643658 (+) 561 WP_002806601.1 D-sedoheptulose 7-phosphate isomerase -
  R8616_RS03200 (B11051_06340) - 643658..644418 (+) 761 Protein_625 glycosyltransferase family 25 protein -
  R8616_RS03205 (B11051_06350) waaF 644411..645355 (-) 945 WP_002844797.1 lipopolysaccharide heptosyltransferase II Regulator
  R8616_RS03210 (B11051_06360) - 645415..646236 (+) 822 WP_002844798.1 glycosyltransferase family 2 protein -
  R8616_RS03215 (B11051_06370) - 646279..647646 (+) 1368 WP_002844799.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  R8616_RS03220 (B11051_06380) gmd 647647..648786 (+) 1140 WP_002844800.1 GDP-mannose 4,6-dehydratase -
  R8616_RS03225 (B11051_06390) - 648779..649717 (+) 939 WP_317649652.1 NAD-dependent epimerase/dehydratase family protein -
  R8616_RS03230 (B11051_06400) - 649710..650879 (+) 1170 WP_002844803.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  R8616_RS03235 (B11051_06410) - 651257..652276 (+) 1020 WP_002844805.1 glycosyltransferase family 10 domain-containing protein -
  R8616_RS03240 (B11051_06420) - 652269..653261 (+) 993 WP_223206634.1 alpha-1,2-fucosyltransferase -
  R8616_RS03245 (B11051_06430) rfbF 653258..654073 (+) 816 WP_002844809.1 glucose-1-phosphate cytidylyltransferase -
  R8616_RS03250 (B11051_06440) rfbG 654077..655150 (+) 1074 WP_002844810.1 CDP-glucose 4,6-dehydratase -
  R8616_RS03255 (B11051_06450) - 655167..656924 (+) 1758 WP_002844812.1 thiamine pyrophosphate-binding protein -
  R8616_RS03260 (B11051_06460) - 656928..657845 (+) 918 WP_002844814.1 NAD-dependent epimerase/dehydratase family protein -
  R8616_RS03265 (B11051_06470) rfbH 657848..659170 (+) 1323 WP_002844816.1 lipopolysaccharide biosynthesis protein RfbH -
  R8616_RS03270 (B11051_06480) - 659186..660259 (+) 1074 WP_002844818.1 glycosyltransferase family 2 protein -
  R8616_RS03275 (B11051_06490) - 660307..661518 (+) 1212 WP_002844820.1 glycosyltransferase family 8 protein -
  R8616_RS03280 (B11051_06500) - 661505..662581 (-) 1077 WP_072225740.1 glycosyltransferase family 4 protein -
  R8616_RS03285 (B11051_06510) - 662578..664125 (-) 1548 WP_002844824.1 glycosyltransferase -
  R8616_RS03290 (B11051_06520) - 664122..665009 (-) 888 WP_002844825.1 lipid A biosynthesis lauroyl acyltransferase -
  R8616_RS03295 (B11051_06530) waaC 665002..666030 (-) 1029 WP_002844827.1 lipopolysaccharide heptosyltransferase I -
  R8616_RS03300 (B11051_06540) - 666095..666886 (+) 792 WP_023362001.1 3'-5' exonuclease -
  R8616_RS03305 (B11051_06550) galE 666943..667929 (+) 987 WP_002844831.1 UDP-glucose 4-epimerase GalE -
  R8616_RS03310 (B11051_06560) - 667923..669623 (+) 1701 WP_002844833.1 ABC transporter ATP-binding protein -
  R8616_RS03315 (B11051_06570) - 669620..670696 (+) 1077 WP_002844835.1 glycosyltransferase -
  R8616_RS03320 (B11051_06580) - 670689..671618 (+) 930 WP_002844836.1 glycosyltransferase family 2 protein -
  R8616_RS03325 (B11051_06590) - 671606..672703 (+) 1098 WP_002844838.1 glycosyltransferase -
  R8616_RS03330 (B11051_06600) - 672707..674851 (+) 2145 WP_002844839.1 STT3 domain-containing protein -
  R8616_RS03335 (B11051_06610) pglA 674863..675993 (+) 1131 WP_002777209.1 N, N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase -
  R8616_RS03340 (B11051_06620) pglC 675986..676588 (+) 603 WP_002777206.1 undecaprenyl phosphate N,N'-diacetylbacillosamine 1-phosphate transferase -
  R8616_RS03345 (B11051_06630) pglD 676575..677162 (+) 588 WP_002780649.1 UDP-N-acetylbacillosamine N-acetyltransferase -
  R8616_RS03350 (B11051_06640) pglE 677317..678477 (+) 1161 WP_002777202.1 UDP-N-acetylbacillosamine transaminase -
  R8616_RS03355 (B11051_06650) pglF 678480..680252 (+) 1773 WP_002777199.1 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 36096.73 Da        Isoelectric Point: 10.3770

>NTDB_id=105148 R8616_RS03205 WP_002844797.1 644411..645355(-) (waaF) [Campylobacter coli strain BCH-11051]
MKIFIHLPTWLGDAVMASPALYGVYHHFKNAEFILYGSFVSTALFKEFPNAKIIVENKKSRYKQTLSLRKKLGKIDLALS
FRSALSSKIILHILKAKKRYFFNKYNFKEEHQVLKYLYFIENSLDIKAHSKDLKLPFRLKFQNPIVLKNGKKILGLNPGA
SFGSAKRWDASYFAQVALNFSKTHEILIFGAGKAEQELCDEIFHILKEKNVKVKNLCNKTTIKMLCQNIIFCDIFITNDS
GPMHIAAAYKTKTIAIFGPTKFTQTSPWQNQNAKLVHLNLACMPCMQKTCPLKHHQCMKDLKPEKILEAIRNFT

Nucleotide


Download         Length: 945 bp        

>NTDB_id=105148 R8616_RS03205 WP_002844797.1 644411..645355(-) (waaF) [Campylobacter coli strain BCH-11051]
ATGAAAATTTTTATCCATCTTCCCACTTGGCTAGGCGATGCAGTCATGGCCTCACCTGCTTTATATGGAGTTTATCATCA
TTTTAAAAATGCTGAATTTATCCTTTATGGATCTTTTGTATCTACAGCGCTTTTTAAGGAATTTCCTAATGCTAAAATCA
TTGTAGAAAATAAAAAATCACGCTACAAACAAACCCTTTCTTTACGCAAAAAACTTGGAAAAATCGATCTAGCCCTTTCT
TTTAGATCAGCTCTTTCCTCTAAGATCATTTTGCATATCCTTAAGGCAAAAAAAAGATATTTTTTTAACAAGTATAATTT
CAAAGAAGAACATCAGGTTTTAAAATATCTTTATTTTATAGAAAATTCACTAGATATCAAAGCTCATTCTAAGGATTTAA
AACTCCCTTTTAGATTAAAATTTCAAAATCCTATTGTTTTAAAAAATGGCAAAAAAATCCTAGGCTTAAATCCTGGAGCA
AGTTTTGGAAGTGCTAAAAGATGGGATGCGAGTTATTTTGCACAAGTTGCTTTAAATTTTAGCAAAACTCATGAAATTTT
GATTTTTGGAGCGGGCAAGGCAGAACAAGAACTTTGCGATGAAATTTTTCATATATTAAAAGAAAAAAATGTCAAGGTAA
AAAATCTTTGCAATAAAACCACCATCAAAATGCTTTGTCAAAATATCATTTTTTGTGATATCTTCATCACAAATGATAGT
GGCCCTATGCACATAGCTGCCGCTTATAAAACAAAAACCATAGCTATCTTTGGGCCTACTAAATTTACCCAAACCTCACC
TTGGCAAAATCAAAATGCAAAATTAGTACATCTAAATTTAGCTTGTATGCCTTGCATGCAAAAAACCTGCCCTTTAAAAC
ATCATCAGTGTATGAAAGATTTAAAACCGGAAAAAATTTTAGAAGCAATCCGAAATTTTACTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(108-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

82.748

99.682

0.825

Multiple sequence alignment