Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEWX4_RS02700 Genome accession   NZ_CP169369
Coordinates   570661..571461 (+) Length   266 a.a.
NCBI ID   WP_104489142.1    Uniprot ID   -
Organism   Acinetobacter indicus strain AIBD14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 565661..576461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEWX4_RS02670 (ACEWX4_02670) - 565992..566411 (+) 420 WP_005182377.1 hypothetical protein -
  ACEWX4_RS02675 (ACEWX4_02675) - 566579..567862 (+) 1284 WP_163142800.1 glycosyltransferase family 1 protein -
  ACEWX4_RS02680 (ACEWX4_02680) - 567895..568446 (+) 552 WP_104470590.1 phosphatase PAP2 family protein -
  ACEWX4_RS02685 (ACEWX4_02685) iscX 568541..568741 (+) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  ACEWX4_RS02690 (ACEWX4_02690) ndk 568851..569282 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  ACEWX4_RS02695 (ACEWX4_02695) rlmN 569405..570640 (+) 1236 WP_180083986.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACEWX4_RS02700 (ACEWX4_02700) pilF 570661..571461 (+) 801 WP_104489142.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACEWX4_RS02705 (ACEWX4_02705) - 571452..572240 (+) 789 WP_016659532.1 helix-turn-helix domain-containing protein -
  ACEWX4_RS02710 (ACEWX4_02710) ispG 572263..573384 (+) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACEWX4_RS02715 (ACEWX4_02715) hisS 573388..574683 (+) 1296 WP_160241611.1 histidine--tRNA ligase -
  ACEWX4_RS02720 (ACEWX4_02720) - 574714..575418 (+) 705 WP_005182401.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30181.36 Da        Isoelectric Point: 9.2581

>NTDB_id=1051053 ACEWX4_RS02700 WP_104489142.1 570661..571461(+) (pilF) [Acinetobacter indicus strain AIBD14]
MRKPSLKFICTSILGLTLVVSGGCQTTGAKGDPEKAVKVRTQLAAEYIKVNNFDAAKRALDQALEINSRDATANMMMGVL
LQQEGSKFNVERAERYFKQALSIEPKNAQARNNYGTYLYQVGRYNDAIEHLQIAGTTLGYDQRFRALENLGRAYVQLGKL
KEAEQAFIQALQINRDAYIAMLELAELKYFNQQQAMATELYEDFVRGVGQNNQDARALWIGIRIARANQDNLGTQVLVNQ
LRALYPESQEYQRYLQLKYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1051053 ACEWX4_RS02700 WP_104489142.1 570661..571461(+) (pilF) [Acinetobacter indicus strain AIBD14]
TTGAGAAAGCCAAGCTTGAAATTTATTTGCACTTCTATTTTGGGGCTGACCTTAGTTGTTTCTGGTGGATGTCAGACTAC
CGGTGCCAAAGGTGATCCAGAAAAAGCAGTAAAGGTAAGAACTCAACTGGCAGCTGAATACATTAAAGTCAATAACTTTG
ATGCAGCGAAACGTGCACTTGATCAGGCACTTGAGATTAATTCTCGCGATGCAACAGCCAATATGATGATGGGCGTGCTG
TTACAGCAAGAAGGAAGCAAGTTTAATGTAGAGCGAGCAGAGCGCTATTTTAAACAGGCACTTTCTATTGAGCCAAAAAA
TGCGCAGGCACGTAACAACTATGGTACGTATTTGTATCAGGTGGGGCGTTATAATGATGCGATTGAACATCTCCAAATAG
CAGGAACAACTTTGGGATATGATCAACGTTTTCGTGCATTGGAAAATTTAGGTCGAGCTTATGTGCAACTGGGTAAATTA
AAAGAGGCGGAACAAGCTTTTATTCAGGCACTACAGATTAATCGTGATGCCTATATTGCTATGCTTGAATTGGCTGAGTT
GAAGTATTTCAATCAGCAACAAGCAATGGCGACAGAACTTTATGAAGATTTTGTACGCGGTGTCGGGCAAAATAATCAAG
ATGCTCGTGCTTTATGGATCGGGATTCGTATTGCTCGAGCAAACCAAGACAATTTGGGGACACAGGTGTTGGTCAATCAA
CTGCGTGCCTTGTATCCAGAGAGTCAGGAATACCAACGTTATCTGCAATTGAAATACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(89-107)

Tetratricopeptide repeat (TPR_16)

(43-81)

Tetratricopeptide repeat (TPR_12)

(108-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.165

100

0.684
  pilF Acinetobacter baylyi ADP1

63.813

96.617

0.617

Multiple sequence alignment