Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACEY2B_RS01140 Genome accession   NZ_CP169315
Coordinates   213224..214585 (+) Length   453 a.a.
NCBI ID   WP_015427149.1    Uniprot ID   -
Organism   Lactococcus lactis strain IFLLLBEK1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 208224..219585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY2B_RS01095 (ACEY2B_01095) - 208515..209162 (+) 648 WP_010906298.1 adenylate kinase -
  ACEY2B_RS01100 (ACEY2B_01100) infA 209459..209677 (+) 219 WP_003130554.1 translation initiation factor IF-1 -
  ACEY2B_RS01105 (ACEY2B_01105) rpmJ 209731..209847 (+) 117 WP_001808836.1 50S ribosomal protein L36 -
  ACEY2B_RS01110 (ACEY2B_01110) rpsM 209865..210230 (+) 366 WP_003130555.1 30S ribosomal protein S13 -
  ACEY2B_RS01115 (ACEY2B_01115) rpsK 210251..210634 (+) 384 WP_010906297.1 30S ribosomal protein S11 -
  ACEY2B_RS01120 (ACEY2B_01120) - 210682..211620 (+) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  ACEY2B_RS01125 (ACEY2B_01125) rplQ 211639..212019 (+) 381 WP_003130558.1 50S ribosomal protein L17 -
  ACEY2B_RS01135 (ACEY2B_01135) - 212835..213062 (+) 228 WP_015427150.1 heavy-metal-associated domain-containing protein -
  ACEY2B_RS01140 (ACEY2B_01140) radA 213224..214585 (+) 1362 WP_015427149.1 DNA repair protein RadA Machinery gene
  ACEY2B_RS01145 (ACEY2B_01145) - 214629..215315 (-) 687 WP_015427148.1 YoaK family protein -
  ACEY2B_RS01150 (ACEY2B_01150) - 215546..216631 (+) 1086 WP_015427147.1 PIN/TRAM domain-containing protein -
  ACEY2B_RS01155 (ACEY2B_01155) - 216696..217325 (+) 630 WP_374788134.1 pyridoxamine 5'-phosphate oxidase family protein -
  ACEY2B_RS01160 (ACEY2B_01160) - 217446..217808 (+) 363 WP_015427145.1 PadR family transcriptional regulator -
  ACEY2B_RS01165 (ACEY2B_01165) - 217795..218142 (+) 348 WP_278217222.1 DUF1048 domain-containing protein -
  ACEY2B_RS01170 (ACEY2B_01170) - 218129..218452 (+) 324 WP_015427143.1 DUF1048 domain-containing protein -
  ACEY2B_RS01175 (ACEY2B_01175) - 218545..219039 (-) 495 WP_015427142.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49907.31 Da        Isoelectric Point: 5.1748

>NTDB_id=1050465 ACEY2B_RS01140 WP_015427149.1 213224..214585(+) (radA) [Lactococcus lactis strain IFLLLBEK1]
MAKKKSSFICQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1050465 ACEY2B_RS01140 WP_015427149.1 213224..214585(+) (radA) [Lactococcus lactis strain IFLLLBEK1]
ATGGCGAAGAAAAAATCATCATTTATTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACTTAGATGAATTTAATCGTGTA
TTAGGAGGGGGTGTTGTTCCTGGTTCATTAGTTTTGATTGGGGGAGACCCTGGGATAGGTAAATCAACTTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGTCGTGTGCTGTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGCTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACCTTTATTGTTGGTCATG
TGACTAAAGAAGGTCAGCTGGCTGGGCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCGACTGGATCTGCGATTG
TTTGTGCACTTGAGGGAACACGGCCCATATTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACTACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACAGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCAGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGGTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.707

100

0.623

Multiple sequence alignment