Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   ACEY2J_RS10075 Genome accession   NZ_CP169293
Coordinates   2022243..2023871 (+) Length   542 a.a.
NCBI ID   WP_001186605.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NSA 739     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2020872..2041257 2022243..2023871 within 0
SCCmec 2015701..2030636 2022243..2023871 within 0


Gene organization within MGE regions


Location: 2015701..2041257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY2J_RS10050 (ACEY2J_10050) - 2015701..2016855 (-) 1155 WP_000912116.1 ATP-binding protein -
  ACEY2J_RS10055 (ACEY2J_10055) - 2017310..2018356 (+) 1047 WP_001042023.1 hypothetical protein -
  ACEY2J_RS10060 (ACEY2J_10060) ssb 2018549..2018845 (+) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  ACEY2J_RS10065 (ACEY2J_10065) cch1 2018845..2020638 (+) 1794 WP_000079474.1 cassette chromosome replicative helicase -
  ACEY2J_RS10070 (ACEY2J_10070) ccrA 2020872..2022221 (+) 1350 WP_000815643.1 cassette chromosome recombinase CcrA Machinery gene
  ACEY2J_RS10075 (ACEY2J_10075) ccrB 2022243..2023871 (+) 1629 WP_001186605.1 cassette chromosome recombinase CcrB Machinery gene
  ACEY2J_RS10080 (ACEY2J_10080) - 2024393..2024743 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ACEY2J_RS10085 (ACEY2J_10085) - 2024830..2025141 (+) 312 WP_000700851.1 DUF960 family protein -
  ACEY2J_RS10090 (ACEY2J_10090) - 2025156..2025662 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  ACEY2J_RS10095 (ACEY2J_10095) - 2025711..2027321 (+) 1611 Protein_1952 IS1182 family transposase -
  ACEY2J_RS10100 (ACEY2J_10100) - 2027312..2027557 (-) 246 Protein_1953 type I restriction enzyme endonuclease domain-containing protein -
  ACEY2J_RS10105 (ACEY2J_10105) - 2027544..2028530 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  ACEY2J_RS10110 (ACEY2J_10110) mecA 2028627..2030636 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  ACEY2J_RS10115 (ACEY2J_10115) - 2030682..2031110 (-) 429 WP_000872606.1 MaoC family dehydratase -
  ACEY2J_RS10120 (ACEY2J_10120) - 2031207..2031950 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  ACEY2J_RS10125 (ACEY2J_10125) - 2032215..2032889 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  ACEY2J_RS10130 (ACEY2J_10130) - 2032955..2033122 (-) 168 Protein_1959 plasmid recombination protein -
  ACEY2J_RS10135 (ACEY2J_10135) catA 2033400..2034047 (-) 648 WP_001010378.1 type A chloramphenicol O-acetyltransferase -
  ACEY2J_RS10140 (ACEY2J_10140) - 2034177..2035112 (-) 936 WP_053023831.1 replication initiation factor domain-containing protein -
  ACEY2J_RS10145 (ACEY2J_10145) mobV 2035639..2036907 (-) 1269 WP_053023830.1 MobV family relaxase -
  ACEY2J_RS10150 (ACEY2J_10150) catA 2037185..2037832 (-) 648 WP_001010378.1 type A chloramphenicol O-acetyltransferase -
  ACEY2J_RS10155 (ACEY2J_10155) - 2037962..2038897 (-) 936 WP_053023831.1 replication initiation factor domain-containing protein -
  ACEY2J_RS10160 (ACEY2J_10160) mobV 2039424..2040518 (-) 1095 Protein_1965 MobV family relaxase -
  ACEY2J_RS10165 (ACEY2J_10165) - 2040583..2041257 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62652.80 Da        Isoelectric Point: 9.8353

>NTDB_id=1050268 ACEY2J_RS10075 WP_001186605.1 2022243..2023871(+) (ccrB) [Staphylococcus aureus strain NSA 739]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
IYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=1050268 ACEY2J_RS10075 WP_001186605.1 2022243..2023871(+) (ccrB) [Staphylococcus aureus strain NSA 739]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTTTCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGATGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAGGTCAAAGATATATACGCTGATCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
ATATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAACGTATTTATGGGTCAAACAAGACGTGCTCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATTGCCAACGCATTGAATCACAAAGGATATGTCACTAAAA
AGGGGAAACCTTTCAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.893

100

0.989

Multiple sequence alignment