Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACETWS_RS27950 Genome accession   NZ_CP169073
Coordinates   6197195..6198811 (-) Length   538 a.a.
NCBI ID   WP_374006398.1    Uniprot ID   -
Organism   Delftia lacustris strain ZWP15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6192195..6203811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACETWS_RS27935 (ACETWS_27935) - 6192792..6193637 (-) 846 WP_374006395.1 hypothetical protein -
  ACETWS_RS27940 (ACETWS_27940) - 6193711..6194850 (-) 1140 WP_374006396.1 slipin family protein -
  ACETWS_RS27945 (ACETWS_27945) rtcR 6195548..6197173 (+) 1626 WP_374006397.1 RNA repair transcriptional activator RtcR -
  ACETWS_RS27950 (ACETWS_27950) rpoN/rpoN1 6197195..6198811 (-) 1617 WP_374006398.1 RNA polymerase factor sigma-54 Machinery gene
  ACETWS_RS27955 (ACETWS_27955) lptB 6198866..6199663 (-) 798 WP_016450697.1 LPS export ABC transporter ATP-binding protein -
  ACETWS_RS27960 (ACETWS_27960) lptA 6199660..6200322 (-) 663 WP_016454602.1 lipopolysaccharide transport periplasmic protein LptA -
  ACETWS_RS27965 (ACETWS_27965) - 6200495..6201145 (-) 651 WP_016450695.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACETWS_RS27970 (ACETWS_27970) - 6201227..6201916 (-) 690 WP_016454601.1 SPOR domain-containing protein -
  ACETWS_RS27975 (ACETWS_27975) argS 6201937..6203634 (-) 1698 WP_374006399.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 538 a.a.        Molecular weight: 59283.33 Da        Isoelectric Point: 4.5762

>NTDB_id=1049794 ACETWS_RS27950 WP_374006398.1 6197195..6198811(-) (rpoN/rpoN1) [Delftia lacustris strain ZWP15]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLGDNPFLELANDDGDHEAASSAPEPATPGADAADSFQESR
ADQPSAAVEWDAPSDTSSGDAVETSGTAESLSWEGDGQTNSGNDDRDWGSDAPSSRTRGQGDDDETDATELARSAESLTD
HLHRQALSLRLGEVDRAALRFLIENLNDDGYLEDPLDELAQGLAGDDLEECEELVHRFTVALRLLQSLEPVGVGARDLAE
CLTLQLRDQLADAEREGDGSAEDEDRQAVLRTALDICAQPMDLLARRDLRKLASLCGAREERLREAMVLIARLEPRPGRR
FADVQRQIVVPDVIVRPVRMGAGRMEFSVQLNPDVMPKLRVHDIYASALRSHRGSEGHAALQQRLQEARWFIKNIQQRFD
TILRVSQAIVERQKNFFVHGELAMRPLVLRDIADALGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTEAGGNASST
AVRALIKQFVAAEDPRKPLSDNQLADMLKEQGIECARRTVAKYRELLKIPPTTLRKAL

Nucleotide


Download         Length: 1617 bp        

>NTDB_id=1049794 ACETWS_RS27950 WP_374006398.1 6197195..6198811(-) (rpoN/rpoN1) [Delftia lacustris strain ZWP15]
TTGAAGCCCGGACTGTCGCTGCGCGTCTCCCAGCATCTTTCGCTCACTCCCCAGCTGCAGCAGTCGATACAACTGCTGCA
GCTGTCCACGCTGGAGCTGGCGCAGGAAGTGGAGCAGATGCTGGGCGACAACCCCTTCCTGGAGCTGGCGAACGACGACG
GCGACCACGAAGCGGCAAGCAGCGCCCCGGAGCCTGCGACGCCCGGCGCCGACGCCGCGGACTCCTTCCAGGAAAGCCGT
GCGGACCAGCCATCGGCTGCGGTGGAATGGGATGCGCCGTCGGACACATCCTCCGGCGATGCCGTCGAGACCTCCGGCAC
GGCCGAATCCCTGAGCTGGGAAGGCGATGGCCAGACCAACTCGGGCAACGACGACCGCGACTGGGGCAGCGATGCGCCTT
CCAGCCGCACGCGCGGCCAGGGTGACGATGACGAGACCGATGCCACCGAGCTGGCACGCAGCGCAGAAAGCCTGACCGAC
CATCTGCACCGCCAGGCCCTGAGCCTGCGCCTGGGCGAGGTGGATCGTGCCGCGCTGCGCTTTCTGATCGAGAACCTCAA
CGACGACGGCTACCTGGAAGACCCGCTCGACGAGCTGGCACAGGGCTTGGCGGGCGACGACCTGGAGGAGTGCGAGGAAC
TGGTGCACCGCTTCACCGTGGCGCTGCGCCTGTTGCAAAGCCTGGAGCCGGTGGGCGTGGGCGCGCGCGACCTGGCCGAA
TGCCTGACGCTGCAGCTGCGCGACCAGCTGGCCGATGCCGAGCGCGAAGGCGACGGCAGTGCCGAGGACGAAGATCGGCA
GGCCGTGCTGCGCACCGCGCTGGACATCTGCGCCCAGCCCATGGACCTGCTGGCGCGGCGTGACCTGCGCAAGCTCGCCA
GCCTGTGCGGCGCGCGCGAGGAGCGGCTGCGCGAGGCGATGGTCCTGATCGCTCGGCTGGAGCCGCGCCCGGGCCGGCGC
TTCGCCGACGTGCAGCGCCAGATCGTCGTGCCCGATGTCATCGTGCGCCCGGTGCGCATGGGTGCGGGACGCATGGAGTT
CAGCGTGCAACTCAACCCCGATGTCATGCCCAAGCTGCGCGTGCACGACATCTATGCCAGCGCGCTGCGCAGCCACCGGG
GCAGCGAAGGCCATGCGGCGTTGCAGCAGCGGCTGCAGGAGGCGCGTTGGTTCATCAAGAACATCCAGCAGCGCTTCGAC
ACCATCCTGCGCGTGTCGCAGGCCATCGTGGAGCGCCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGCCCGCT
GGTGCTGCGCGACATTGCCGATGCGCTGGGCCTGCATGAATCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCA
CGCCGCAGGGCACCTACGAACTCAAGTATTTCTTCGGCTCGGGCCTGGGCACCGAGGCGGGCGGCAACGCGTCGAGCACG
GCCGTGCGCGCGCTCATCAAGCAGTTCGTCGCAGCCGAGGACCCCAGGAAGCCGCTGTCGGACAACCAGCTCGCCGACAT
GCTCAAGGAGCAGGGCATCGAATGCGCACGGCGCACCGTGGCCAAGTACCGCGAACTGCTCAAGATCCCCCCGACCACGC
TGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-365)

Sigma-54, DNA binding domain (Sigma54_DBD)

(380-536)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.111

100

0.513

Multiple sequence alignment