Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | ACETWW_RS08755 | Genome accession | NZ_CP169072 |
| Coordinates | 1801852..1802286 (+) | Length | 144 a.a. |
| NCBI ID | WP_136396382.1 | Uniprot ID | - |
| Organism | Bacillus velezensis strain T971 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 1803211..1817027 | 1801852..1802286 | flank | 925 |
| IScluster/Tn | 1803211..1804361 | 1801852..1802286 | flank | 925 |
Gene organization within MGE regions
Location: 1801852..1817027
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACETWW_RS08755 (ACETWW_08745) | nucA/comI | 1801852..1802286 (+) | 435 | WP_136396382.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| ACETWW_RS08760 (ACETWW_08750) | - | 1802346..1803101 (+) | 756 | WP_003154084.1 | YoaK family protein | - |
| ACETWW_RS08765 (ACETWW_08755) | - | 1803211..1804361 (+) | 1151 | WP_374004927.1 | IS3 family transposase | - |
| ACETWW_RS08770 (ACETWW_08760) | - | 1804423..1804785 (-) | 363 | WP_007410383.1 | hypothetical protein | - |
| ACETWW_RS08775 (ACETWW_08765) | - | 1804978..1806306 (-) | 1329 | WP_374004928.1 | S8 family peptidase | - |
| ACETWW_RS08780 (ACETWW_08770) | - | 1806485..1806718 (+) | 234 | WP_015239897.1 | hypothetical protein | - |
| ACETWW_RS08785 (ACETWW_08775) | - | 1806974..1807681 (+) | 708 | WP_007410381.1 | poly-gamma-glutamate hydrolase family protein | - |
| ACETWW_RS08790 (ACETWW_08780) | - | 1807741..1808193 (+) | 453 | WP_014305039.1 | OsmC family protein | - |
| ACETWW_RS08795 (ACETWW_08785) | - | 1808207..1808560 (-) | 354 | WP_007410379.1 | DMT family transporter | - |
| ACETWW_RS08800 (ACETWW_08790) | - | 1808578..1808892 (-) | 315 | WP_007410378.1 | DMT family transporter | - |
| ACETWW_RS08805 (ACETWW_08795) | - | 1809030..1809347 (-) | 318 | WP_032866021.1 | hypothetical protein | - |
| ACETWW_RS08810 (ACETWW_08800) | - | 1809449..1809853 (+) | 405 | WP_014417831.1 | YmaF family protein | - |
| ACETWW_RS08815 (ACETWW_08805) | miaA | 1809952..1810896 (+) | 945 | WP_007410375.1 | tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA | - |
| ACETWW_RS08820 (ACETWW_08810) | hfq | 1810936..1811157 (+) | 222 | WP_003154064.1 | RNA chaperone Hfq | - |
| ACETWW_RS08825 (ACETWW_08815) | - | 1811256..1811531 (+) | 276 | WP_025284768.1 | YmzC family protein | - |
| ACETWW_RS08830 (ACETWW_08820) | - | 1811614..1811829 (+) | 216 | WP_003154062.1 | hypothetical protein | - |
| ACETWW_RS08835 (ACETWW_08825) | nrdI | 1812089..1812481 (+) | 393 | WP_003154061.1 | class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI | - |
| ACETWW_RS08840 (ACETWW_08830) | nrdE | 1812441..1814543 (+) | 2103 | WP_007611605.1 | class 1b ribonucleoside-diphosphate reductase subunit alpha | - |
| ACETWW_RS08845 (ACETWW_08835) | nrdF | 1814561..1815550 (+) | 990 | WP_012117608.1 | class 1b ribonucleoside-diphosphate reductase subunit beta | - |
| ACETWW_RS08850 (ACETWW_08840) | - | 1815599..1816219 (+) | 621 | WP_012117609.1 | hypothetical protein | - |
| ACETWW_RS08855 (ACETWW_08845) | - | 1816269..1817027 (-) | 759 | WP_012117610.1 | N-acetylmuramoyl-L-alanine amidase | - |
Sequence
Protein
Download Length: 144 a.a. Molecular weight: 15544.47 Da Isoelectric Point: 7.2418
>NTDB_id=1049734 ACETWW_RS08755 WP_136396382.1 1801852..1802286(+) (nucA/comI) [Bacillus velezensis strain T971]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISTGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISTGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ
Nucleotide
Download Length: 435 bp
>NTDB_id=1049734 ACETWW_RS08755 WP_136396382.1 1801852..1802286(+) (nucA/comI) [Bacillus velezensis strain T971]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGACAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGACAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
57.258 |
86.111 |
0.493 |