Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEQ85_RS05355 Genome accession   NZ_CP168966
Coordinates   1127846..1128643 (-) Length   265 a.a.
NCBI ID   WP_373883483.1    Uniprot ID   -
Organism   Acinetobacter pittii strain LS-AP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1122846..1133643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ85_RS05335 (ACEQ85_05335) - 1123911..1124609 (-) 699 WP_017398816.1 tetratricopeptide repeat protein -
  ACEQ85_RS05340 (ACEQ85_05340) hisS 1124638..1125930 (-) 1293 WP_017385736.1 histidine--tRNA ligase -
  ACEQ85_RS05345 (ACEQ85_05345) ispG 1125927..1127042 (-) 1116 WP_284086406.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACEQ85_RS05350 (ACEQ85_05350) - 1127064..1127855 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  ACEQ85_RS05355 (ACEQ85_05355) pilF 1127846..1128643 (-) 798 WP_373883483.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACEQ85_RS05360 (ACEQ85_05360) rlmN 1128658..1129893 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACEQ85_RS05365 (ACEQ85_05365) ndk 1130018..1130449 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  ACEQ85_RS05370 (ACEQ85_05370) iscX 1130563..1130763 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  ACEQ85_RS05375 (ACEQ85_05375) - 1130863..1131414 (-) 552 WP_373883484.1 phosphatase PAP2 family protein -
  ACEQ85_RS05380 (ACEQ85_05380) - 1131439..1132728 (-) 1290 WP_373883738.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29988.22 Da        Isoelectric Point: 9.0417

>NTDB_id=1048970 ACEQ85_RS05355 WP_373883483.1 1127846..1128643(-) (pilF) [Acinetobacter pittii strain LS-AP]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRTLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQL
RALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=1048970 ACEQ85_RS05355 WP_373883483.1 1127846..1128643(-) (pilF) [Acinetobacter pittii strain LS-AP]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCAGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ATTCAGCAAAGCGAACTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
TTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGGGTG
CAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAGTTA
CGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(89-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.943
  pilF Acinetobacter baylyi ADP1

75.769

98.113

0.743

Multiple sequence alignment