Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   R8605_RS06105 Genome accession   NZ_AP028358
Coordinates   1174953..1175906 (+) Length   317 a.a.
NCBI ID   WP_002839071.1    Uniprot ID   A0A5Z1EHW0
Organism   Campylobacter jejuni strain BCH-10569     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1156348..1179556 1174953..1175906 within 0


Gene organization within MGE regions


Location: 1156348..1179556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8605_RS06010 (B10569_12040) pglI 1156348..1157277 (-) 930 WP_002882940.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  R8605_RS06015 (B10569_12050) pglH 1157270..1158349 (-) 1080 WP_308042762.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  R8605_RS06020 (B10569_12060) pglK 1158346..1160040 (-) 1695 WP_308042761.1 ABC-type lipopolysaccharide transporter PglK -
  R8605_RS06025 (B10569_12070) galE 1160034..1161020 (-) 987 WP_306197866.1 UDP-glucose 4-epimerase GalE -
  R8605_RS06030 (B10569_12080) - 1161078..1161872 (-) 795 WP_002883266.1 3'-5' exonuclease -
  R8605_RS06035 (B10569_12090) waaC 1161938..1162966 (+) 1029 WP_306179559.1 lipopolysaccharide heptosyltransferase I -
  R8605_RS06040 (B10569_12100) htrB 1162956..1163843 (+) 888 WP_070296704.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  R8605_RS06045 (B10569_12110) - 1163840..1165387 (+) 1548 WP_306181089.1 glycosyltransferase family 2 protein -
  R8605_RS06050 (B10569_12130) - 1165391..1166561 (+) 1171 Protein_1168 glycosyltransferase family 2 protein -
  R8605_RS06055 (B10569_12140) - 1166609..1167670 (+) 1062 WP_002882966.1 beta-1,4-N-acetylgalactosaminyltransferase -
  R8605_RS06060 - 1167642..1168539 (-) 898 Protein_1170 glycosyltransferase family 2 protein -
  R8605_RS06065 (B10569_12170) - 1168628..1169506 (+) 879 WP_306179556.1 alpha-2,3-sialyltransferase -
  R8605_RS06070 (B10569_12180) neuB 1169507..1170547 (+) 1041 WP_070284287.1 N-acetylneuraminate synthase -
  R8605_RS06075 (B10569_12190) neuC 1170544..1171668 (+) 1125 WP_308042760.1 UDP-N-acetylglucosamine 2-epimerase -
  R8605_RS06080 (B10569_12200) - 1171661..1172278 (+) 618 WP_167562429.1 glycosyltransferase -
  R8605_RS06085 (B10569_12210) - 1172275..1172607 (+) 333 WP_019260376.1 hypothetical protein -
  R8605_RS06090 (B10569_12220) - 1172604..1173269 (+) 666 WP_002869017.1 cytidylyltransferase domain-containing protein -
  R8605_RS06095 (B10569_12230) - 1173257..1174090 (+) 834 WP_002882959.1 CatB-related O-acetyltransferase -
  R8605_RS06100 (B10569_12240) waaV 1174082..1174894 (-) 813 WP_306179553.1 glycosyltransferase family 2 protein -
  R8605_RS06105 (B10569_12250) waaF 1174953..1175906 (+) 954 WP_002839071.1 glycosyltransferase family 9 protein Regulator
  R8605_RS06110 (B10569_12260) - 1175913..1176668 (-) 756 WP_002869013.1 glycosyltransferase family 25 protein -
  R8605_RS06115 (B10569_12270) gmhA 1176668..1177228 (-) 561 WP_308042759.1 D-sedoheptulose 7-phosphate isomerase -
  R8605_RS06120 (B10569_12280) rfaE1 1177225..1178610 (-) 1386 WP_107701925.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  R8605_RS06125 (B10569_12290) rfaD 1178603..1179556 (-) 954 WP_052802041.1 ADP-glyceromanno-heptose 6-epimerase -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 36557.15 Da        Isoelectric Point: 10.2875

>NTDB_id=104727 R8605_RS06105 WP_002839071.1 1174953..1175906(+) (waaF) [Campylobacter jejuni strain BCH-10569]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDVSYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENARWVHLNLACMPCMQKTCPLKHHKCMKDLKPEIIIEVSKQLLFLN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=104727 R8605_RS06105 WP_002839071.1 1174953..1175906(+) (waaF) [Campylobacter jejuni strain BCH-10569]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCGCCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGCAAAATCGATTTTAGCTTTGCT
TTTAGGTCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACGAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGTGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGTCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAGATGGGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAACCAGAAATCATCATAGAAGTAAGTAAACAATTGCTATTTCTAAATTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Z1EHW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.631

99.054

0.927

Multiple sequence alignment