Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   R8859_RS09530 Genome accession   NZ_OY754407
Coordinates   1962962..1963954 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli isolate F247     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 1957962..1968954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8859_RS09505 (FGAF247_18200) ygbN 1958617..1959981 (+) 1365 WP_000104439.1 GntP family transporter -
  R8859_RS09510 (FGAF247_18210) - 1960020..1960256 (-) 237 WP_000562984.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  R8859_RS09515 (FGAF247_18220) - 1960267..1961694 (-) 1428 WP_000863223.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  R8859_RS09520 (FGAF247_18230) - 1961694..1962287 (-) 594 WP_000767707.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  R8859_RS09525 (FGAF247_18240) - 1962434..1962841 (+) 408 WP_001208070.1 MarR family winged helix-turn-helix transcriptional regulator -
  R8859_RS09530 (FGAF247_18250) rpoS 1962962..1963954 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  R8859_RS09535 (FGAF247_18260) nlpD 1964017..1965111 (-) 1095 WP_306174120.1 murein hydrolase activator NlpD -
  R8859_RS09540 (FGAF247_18270) pcm 1965296..1965922 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  R8859_RS09545 (FGAF247_18280) surE 1965916..1966677 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  R8859_RS09550 (FGAF247_18290) truD 1966658..1967707 (-) 1050 WP_000568943.1 tRNA pseudouridine(13) synthase TruD -
  R8859_RS09555 (FGAF247_18300) ispF 1967704..1968183 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  R8859_RS09560 (FGAF247_18310) ispD 1968183..1968893 (-) 711 WP_000246136.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=1046580 R8859_RS09530 WP_000081550.1 1962962..1963954(-) (rpoS) [Escherichia coli isolate F247]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=1046580 R8859_RS09530 WP_000081550.1 1962962..1963954(-) (rpoS) [Escherichia coli isolate F247]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715