Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   R8858_RS00435 Genome accession   NZ_OY754398
Coordinates   85496..86164 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli isolate F437     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 80496..91164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8858_RS00405 (FGAF437_00780) yicC 80515..81378 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  R8858_RS00410 (FGAF437_00790) rph 81505..82221 (+) 717 WP_001247093.1 ribonuclease PH -
  R8858_RS00415 (FGAF437_00800) pyrE 82287..82928 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  R8858_RS00420 (FGAF437_00810) slmA 82965..83561 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  R8858_RS00425 (FGAF437_00820) dut 83668..84126 (-) 459 WP_000976070.1 dUTP diphosphatase -
  R8858_RS00430 (FGAF437_00830) coaBC 84104..85324 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  R8858_RS00435 (FGAF437_00840) radC 85496..86164 (+) 669 WP_001298959.1 RadC family protein Machinery gene
  R8858_RS00440 (FGAF437_00850) rpmB 86381..86617 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  R8858_RS00445 (FGAF437_00860) rpmG 86638..86805 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  R8858_RS00450 (FGAF437_00870) mutM 86903..87712 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  R8858_RS00455 (FGAF437_00880) coaD 87751..88230 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  R8858_RS00460 (FGAF437_00890) waaA 88238..89515 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  R8858_RS00465 (FGAF437_00900) rfaQ 89928..90986 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=1046475 R8858_RS00435 WP_001298959.1 85496..86164(+) (radC) [Escherichia coli isolate F437]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=1046475 R8858_RS00435 WP_001298959.1 85496..86164(+) (radC) [Escherichia coli isolate F437]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496