Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   NTHI477_RS06850 Genome accession   NZ_CP007470
Coordinates   1426443..1428635 (-) Length   730 a.a.
NCBI ID   WP_044332470.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 477     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1421443..1433635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI477_RS06825 (NTHI477_01378) relA 1421737..1423968 (+) 2232 WP_013527485.1 GTP diphosphokinase -
  NTHI477_RS06830 (NTHI477_01379) - 1423983..1424339 (+) 357 WP_005632174.1 diacylglycerol kinase -
  NTHI477_RS06835 (NTHI477_01380) mog 1424422..1425015 (+) 594 WP_005635167.1 molybdopterin adenylyltransferase -
  NTHI477_RS06840 (NTHI477_01381) glnB 1425017..1425355 (+) 339 WP_005649085.1 nitrogen regulatory protein P-II -
  NTHI477_RS06845 (NTHI477_01382) - 1425355..1426401 (+) 1047 WP_005649088.1 AI-2E family transporter -
  NTHI477_RS06850 (NTHI477_01383) priA 1426443..1428635 (-) 2193 WP_044332470.1 primosomal protein N' Machinery gene
  NTHI477_RS06855 (NTHI477_01384) trmB 1428720..1429460 (+) 741 WP_005656330.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  NTHI477_RS06860 (NTHI477_01385) - 1429545..1429889 (+) 345 WP_005656331.1 YggL family protein -
  NTHI477_RS06865 (NTHI477_01386) napF 1430095..1430625 (+) 531 WP_005656332.1 ferredoxin-type protein NapF -
  NTHI477_RS06870 (NTHI477_01387) - 1430618..1430899 (+) 282 WP_005659946.1 chaperone NapD -
  NTHI477_RS06875 (NTHI477_01388) napA 1430936..1433419 (+) 2484 WP_044332473.1 nitrate reductase catalytic subunit NapA -

Sequence

Protein


Download         Length: 730 a.a.        Molecular weight: 82291.54 Da        Isoelectric Point: 9.3184

>NTDB_id=104640 NTHI477_RS06850 WP_044332470.1 1426443..1428635(-) (priA) [Haemophilus influenzae strain 477]
MKIVRVALAVPLPRLFDYFVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPVFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNVLKQGELKRSKKQAEALQYLSETDLEKGNNNFSSA
IWSALKAKGFIEEITIHTNPLSWQQRLGNNPIVNAENRLTLNKQQALAFSQLLFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEQDASYKQQDSWRYHARDLAIVLAQKLNIAVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQNI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTARKGKLEGYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALF
KAQARHSDLAENCLSQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSLVRL
ILDVDPQDLS

Nucleotide


Download         Length: 2193 bp        

>NTDB_id=104640 NTHI477_RS06850 WP_044332470.1 1426443..1428635(-) (priA) [Haemophilus influenzae strain 477]
ATGAAAATTGTCCGAGTGGCACTGGCTGTGCCGCTTCCTCGTTTATTTGATTATTTCGTCCCCGATGATGTTTCATTACA
AATCGGTATGCGTGTTCTTGTGCCTTTTGGCACCCAAAAACGTGTCGCCATTGTGGCTGATTTTCCGACAAAATCGGACG
TGCCAGAGGATAAACTCAAGGCTATTCTTCAACCATTGGATTTAGCCCCAGTTTTTACGCCCATTTATTGGGATTGGCTT
CATTGGGCAGCAAATTATTATCAAGCGGGATTAGGCGATGTGTTATTTCAAGCCTTGCCTGTGAAATTGCGTAACGGCGA
AAGTGCGGTAAAAAATGACCGCACTTTTTGGCGGATTACGGATGCGGGGAAAAATGTCCTTAAACAAGGCGAATTAAAAC
GCTCGAAAAAGCAAGCTGAAGCCCTACAATACTTATCTGAAACTGATCTTGAAAAAGGCAATAATAATTTTTCCTCTGCC
ATTTGGTCAGCCTTAAAAGCCAAAGGGTTTATTGAGGAAATTACTATCCACACAAATCCCCTAAGCTGGCAACAAAGATT
AGGCAATAACCCAATTGTCAATGCTGAAAATCGTTTAACCTTAAATAAGCAGCAAGCCTTAGCGTTTAGCCAATTGCTCT
TTCATTCTGGTTTCAATGTATGGCTGCTCGATGGCGTGACTGGTTCAGGTAAAACAGAAATTTATCTGCAATATATTGAA
GAAATTTTAAAATCGGGTAAGCAAGTATTAGTGCTTGTACCCGAAATCGGGCTAACACCTCAAACGGTGCAACGTTTCAA
AGCACGTTTCAACGTAGAAATTGATGTACTGCATTCTAATTTAACCGATACGCAACGGCTTTATGTATGGGATCGCGCCC
GCTCTGGACAAAGTGCCATTGTGATTGGCACGAGATCGGCATTGTTCACGCAATTTTCTAATCTTGGTGCAATTATTTTG
GACGAAGAACAGGATGCGTCTTACAAACAGCAAGACAGTTGGCGTTACCATGCGCGAGACTTGGCGATTGTGCTGGCTCA
AAAACTAAATATTGCGGTATTAATGGGCTCCGCCACGCCAAGTTTAGAAAGTATTAATAATGTACAAAACGGCAAATACC
AACATTTAGTTTTATCAAAAAGAGCGGGAAATTCTACCGCACTTCGTCATTTCGTTATTGATTTGAAAAATCAAAATATA
CAAAACGGGTTATCAAAACCCTTGTTGGAACGAATGAAAGCCCATCTCGAAAAAGGCAATCAAGTGTTGCTTTTCTTAAA
TCGTCGTGGCTTTGCACCCGTATTGCTTTGCCATGAATGTGGTTGGATCGCACAATGCCCTCATTGTGAAAAACCTTATA
CCTATCATCAGCATCAAAATGTATTGCGTTGTCATCATTGTGGCGCACAAAAAACTATTCCTCGCCAATGTGGCGATTGC
GGTTCTACCCATTTAGTTACTACTGGTCTAGGCACAGAACAATTAGAAGAAACCTTGAAAACGCTATTTCCTCATTATTC
TGTAGCGCGAATCGACCGCGATAGCACGGCACGTAAAGGAAAATTGGAAGGTTATTTAGAAGATATTCAACAAGGCAAAA
GCCAAATTTTGATTGGCACACAAATGCTCGCGAAAGGACATCATTTTCCGAATGTTACTCTCGTTGCCTTAGTGAATGTA
GATAGTGCATTATTTTCACTGGATTTTCGTGCAGAAGAACGTTTAGCTCAACTTTATATTCAAGTTGCTGGACGTGCAGG
ACGTGCCGATAAACAAGGTGAAGTTGTGCTACAAACACATTATCCTGATCATCCTTTGCTTACCACTTTATTGGCAAATG
GCTACCAAGCTTTTGCAAAAGAAACCTTACAATTACGCCATTCAATGGGATTGCCGCCTTTTACTTTTCAGGCGTTATTC
AAAGCACAGGCTCGACATTCTGATCTTGCAGAAAATTGCTTAAGCCAAATTGCAGATTTTTTTCAATCGAAGCAAATTAC
GGGATTACAAATGCTTGGCCCAATGCCTGCACCGTTCAGCAAAAAAGCGGGGCAATATCGCTGGCAGTTATTATTGCAAC
ATCCGTCAAGAATGACGTTGCAAAAAGCATTAAGAGAATATCAACAAGCCGAACTTGAAAAAAACAGCCTAGTGCGGTTA
ATTTTGGACGTCGATCCGCAAGACTTATCATAA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(503-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-100)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-471)

Primosomal protein N C-terminal domain (PriA_C)

(633-726)

DEAD/DEAH box helicase (DEAD)

(204-366)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.624

100

0.455