Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEQ38_RS15055 Genome accession   NZ_CP168747
Coordinates   3254577..3256076 (-) Length   499 a.a.
NCBI ID   WP_078222387.1    Uniprot ID   -
Organism   Ralstonia syzygii subsp. celebesensis strain NCPPB 3727     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3249577..3261076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ38_RS15025 (ACEQ38_15025) - 3250090..3250740 (-) 651 WP_013213616.1 LON peptidase substrate-binding domain-containing protein -
  ACEQ38_RS15030 (ACEQ38_15030) rapZ 3250820..3251710 (-) 891 WP_013213615.1 RNase adapter RapZ -
  ACEQ38_RS15035 (ACEQ38_15035) - 3251803..3252132 (-) 330 WP_013213614.1 PsiF family protein -
  ACEQ38_RS15040 (ACEQ38_15040) hprK 3252324..3253298 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACEQ38_RS15045 (ACEQ38_15045) ptsN 3253369..3253824 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEQ38_RS15050 (ACEQ38_15050) hpf 3254172..3254525 (-) 354 WP_013213613.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEQ38_RS15055 (ACEQ38_15055) rpoN/rpoN1 3254577..3256076 (-) 1500 WP_078222387.1 RNA polymerase factor sigma-54 Machinery gene
  ACEQ38_RS15060 (ACEQ38_15060) lptB 3256174..3256962 (-) 789 WP_013213611.1 LPS export ABC transporter ATP-binding protein -
  ACEQ38_RS15065 (ACEQ38_15065) lptA 3256996..3257592 (-) 597 WP_013213610.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEQ38_RS15070 (ACEQ38_15070) lptC 3257661..3258293 (-) 633 WP_013213609.1 LPS export ABC transporter periplasmic protein LptC -
  ACEQ38_RS15075 (ACEQ38_15075) - 3258295..3258882 (-) 588 WP_013213608.1 HAD family hydrolase -
  ACEQ38_RS15080 (ACEQ38_15080) - 3258915..3259898 (-) 984 WP_013213607.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55061.45 Da        Isoelectric Point: 4.8496

>NTDB_id=1045477 ACEQ38_RS15055 WP_078222387.1 3254577..3256076(-) (rpoN/rpoN1) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGVDGSVSALQSSSATPPPTE
SPPPAANGADHADTAEASYENGSDFDSDYGADRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSLR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEAELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGSANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMAPPLGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1045477 ACEQ38_RS15055 WP_078222387.1 3254577..3256076(-) (rpoN/rpoN1) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCAGCGCGCATCGGCGTCGACGGCTCGGTCAGTGCCCTGCAGAGCAGCAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCACGCCGACACTGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTATGGCGCCGATCGTTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCGCAGTCCGACGAAGACGATCGCGCCC
CGATGCAGCTGCGCGAGGCCGAACCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCCTGCGC
GACAAAGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCTGAACTCGAGGCCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGTTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGTCTGAAGAAAGCGCTGTCCGTCGATGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCCCCGTTCCCTGGCCACGCCTTCGGGCGTGCCGAGGCCGACT
TTGTCGTGCCCGACGTGGTGGTGCGTAAAACTGCCGCAGGCTGGATGGCGCAGTTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATTCTGCGCAGCAGCCGAGGCGAATCGGGCTCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCTCGCTGGCTGATCAAGAACATCCAACAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGCCCCCCCTGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.194

100

0.972

Multiple sequence alignment