Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEPM4_RS02110 Genome accession   NZ_CP168733
Coordinates   451716..453215 (-) Length   499 a.a.
NCBI ID   WP_064477487.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN5935     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446716..458215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM4_RS02080 (ACEPM4_02080) - 447224..447874 (-) 651 WP_011000364.1 LON peptidase substrate-binding domain-containing protein -
  ACEPM4_RS02085 (ACEPM4_02085) rapZ 447952..448842 (-) 891 WP_011000365.1 RNase adapter RapZ -
  ACEPM4_RS02090 (ACEPM4_02090) - 448936..449271 (-) 336 WP_043876703.1 PsiF family protein -
  ACEPM4_RS02095 (ACEPM4_02095) hprK 449462..450436 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACEPM4_RS02100 (ACEPM4_02100) ptsN 450507..450962 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEPM4_RS02105 (ACEPM4_02105) hpf 451311..451664 (-) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEPM4_RS02110 (ACEPM4_02110) rpoN/rpoN1 451716..453215 (-) 1500 WP_064477487.1 RNA polymerase factor sigma-54 Machinery gene
  ACEPM4_RS02115 (ACEPM4_02115) lptB 453313..454101 (-) 789 WP_019717491.1 LPS export ABC transporter ATP-binding protein -
  ACEPM4_RS02120 (ACEPM4_02120) lptA 454135..454710 (-) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEPM4_RS02125 (ACEPM4_02125) lptC 454779..455411 (-) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  ACEPM4_RS02130 (ACEPM4_02130) - 455413..456000 (-) 588 WP_011000373.1 HAD family hydrolase -
  ACEPM4_RS02135 (ACEPM4_02135) - 456039..457022 (-) 984 WP_016724265.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55257.58 Da        Isoelectric Point: 4.8064

>NTDB_id=1045378 ACEPM4_RS02110 WP_064477487.1 451716..453215(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN5935]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHTFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1045378 ACEPM4_RS02110 WP_064477487.1 451716..453215(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN5935]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGTTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACACCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998

Multiple sequence alignment