Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEPM0_RS15145 Genome accession   NZ_CP168731
Coordinates   3194870..3196369 (+) Length   499 a.a.
NCBI ID   WP_011000369.1    Uniprot ID   A0A223GJ62
Organism   Ralstonia pseudosolanacearum strain RUN6285     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3189870..3201369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM0_RS15120 (ACEPM0_15120) - 3191063..3192046 (+) 984 WP_197345549.1 SIS domain-containing protein -
  ACEPM0_RS15125 (ACEPM0_15125) - 3192085..3192672 (+) 588 WP_011000373.1 HAD family hydrolase -
  ACEPM0_RS15130 (ACEPM0_15130) lptC 3192674..3193306 (+) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  ACEPM0_RS15135 (ACEPM0_15135) lptA 3193375..3193950 (+) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEPM0_RS15140 (ACEPM0_15140) lptB 3193984..3194772 (+) 789 WP_019717491.1 LPS export ABC transporter ATP-binding protein -
  ACEPM0_RS15145 (ACEPM0_15145) rpoN/rpoN1 3194870..3196369 (+) 1500 WP_011000369.1 RNA polymerase factor sigma-54 Machinery gene
  ACEPM0_RS15150 (ACEPM0_15150) hpf 3196421..3196774 (+) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEPM0_RS15155 (ACEPM0_15155) ptsN 3197123..3197578 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEPM0_RS15160 (ACEPM0_15160) hprK 3197649..3198623 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACEPM0_RS15165 (ACEPM0_15165) - 3198814..3199149 (+) 336 WP_020425146.1 PsiF family protein -
  ACEPM0_RS15170 (ACEPM0_15170) rapZ 3199243..3200133 (+) 891 WP_028852570.1 RNase adapter RapZ -
  ACEPM0_RS15175 (ACEPM0_15175) - 3200211..3200861 (+) 651 WP_028852569.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55227.56 Da        Isoelectric Point: 4.8064

>NTDB_id=1045372 ACEPM0_RS15145 WP_011000369.1 3194870..3196369(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6285]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1045372 ACEPM0_RS15145 WP_011000369.1 3194870..3196369(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6285]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGTGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GJ62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

100

100

1

Multiple sequence alignment