Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEPMY_RS15150 Genome accession   NZ_CP168729
Coordinates   3194871..3196370 (+) Length   499 a.a.
NCBI ID   WP_011000369.1    Uniprot ID   A0A223GJ62
Organism   Ralstonia pseudosolanacearum strain RUN6340     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3189871..3201370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPMY_RS15125 (ACEPMY_15125) - 3191064..3192047 (+) 984 WP_197345549.1 SIS domain-containing protein -
  ACEPMY_RS15130 (ACEPMY_15130) - 3192086..3192673 (+) 588 WP_011000373.1 HAD family hydrolase -
  ACEPMY_RS15135 (ACEPMY_15135) lptC 3192675..3193307 (+) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  ACEPMY_RS15140 (ACEPMY_15140) lptA 3193376..3193951 (+) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEPMY_RS15145 (ACEPMY_15145) lptB 3193985..3194773 (+) 789 WP_019717491.1 LPS export ABC transporter ATP-binding protein -
  ACEPMY_RS15150 (ACEPMY_15150) rpoN/rpoN1 3194871..3196370 (+) 1500 WP_011000369.1 RNA polymerase factor sigma-54 Machinery gene
  ACEPMY_RS15155 (ACEPMY_15155) hpf 3196422..3196775 (+) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEPMY_RS15160 (ACEPMY_15160) ptsN 3197124..3197579 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEPMY_RS15165 (ACEPMY_15165) hprK 3197650..3198624 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACEPMY_RS15170 (ACEPMY_15170) - 3198815..3199150 (+) 336 WP_020425146.1 PsiF family protein -
  ACEPMY_RS15175 (ACEPMY_15175) rapZ 3199244..3200134 (+) 891 WP_028852570.1 RNase adapter RapZ -
  ACEPMY_RS15180 (ACEPMY_15180) - 3200212..3200862 (+) 651 WP_028852569.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55227.56 Da        Isoelectric Point: 4.8064

>NTDB_id=1045351 ACEPMY_RS15150 WP_011000369.1 3194871..3196370(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6340]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1045351 ACEPMY_RS15150 WP_011000369.1 3194871..3196370(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6340]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGTGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GJ62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

100

100

1

Multiple sequence alignment