Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEPM3_RS02205 Genome accession   NZ_CP168727
Coordinates   473859..475358 (-) Length   499 a.a.
NCBI ID   WP_247416921.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN6499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468859..480358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM3_RS02175 (ACEPM3_02175) - 469367..470017 (-) 651 WP_011000364.1 LON peptidase substrate-binding domain-containing protein -
  ACEPM3_RS02180 (ACEPM3_02180) rapZ 470095..470985 (-) 891 WP_011000365.1 RNase adapter RapZ -
  ACEPM3_RS02185 (ACEPM3_02185) - 471079..471414 (-) 336 WP_043876703.1 PsiF family protein -
  ACEPM3_RS02190 (ACEPM3_02190) hprK 471605..472579 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACEPM3_RS02195 (ACEPM3_02195) ptsN 472650..473105 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEPM3_RS02200 (ACEPM3_02200) hpf 473454..473807 (-) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEPM3_RS02205 (ACEPM3_02205) rpoN/rpoN1 473859..475358 (-) 1500 WP_247416921.1 RNA polymerase factor sigma-54 Machinery gene
  ACEPM3_RS02210 (ACEPM3_02210) lptB 475456..476244 (-) 789 WP_016724264.1 LPS export ABC transporter ATP-binding protein -
  ACEPM3_RS02215 (ACEPM3_02215) lptA 476278..476853 (-) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEPM3_RS02220 (ACEPM3_02220) lptC 476922..477554 (-) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  ACEPM3_RS02225 (ACEPM3_02225) - 477556..478143 (-) 588 WP_011000373.1 HAD family hydrolase -
  ACEPM3_RS02230 (ACEPM3_02230) - 478182..479165 (-) 984 WP_016724265.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55200.53 Da        Isoelectric Point: 4.8064

>NTDB_id=1045315 ACEPM3_RS02205 WP_247416921.1 473859..475358(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6499]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVSQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1045315 ACEPM3_RS02205 WP_247416921.1 473859..475358(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6499]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGTGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAGCCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998

Multiple sequence alignment