Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEPM5_RS02065 Genome accession   NZ_CP168725
Coordinates   436861..438360 (-) Length   499 a.a.
NCBI ID   WP_011000369.1    Uniprot ID   A0A223GJ62
Organism   Ralstonia pseudosolanacearum strain RUN6534     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431861..443360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM5_RS02035 (ACEPM5_02035) - 432369..433019 (-) 651 WP_011000364.1 LON peptidase substrate-binding domain-containing protein -
  ACEPM5_RS02040 (ACEPM5_02040) rapZ 433097..433987 (-) 891 WP_011000365.1 RNase adapter RapZ -
  ACEPM5_RS02045 (ACEPM5_02045) - 434081..434416 (-) 336 WP_043876703.1 PsiF family protein -
  ACEPM5_RS02050 (ACEPM5_02050) hprK 434607..435581 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACEPM5_RS02055 (ACEPM5_02055) ptsN 435652..436107 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEPM5_RS02060 (ACEPM5_02060) hpf 436456..436809 (-) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEPM5_RS02065 (ACEPM5_02065) rpoN/rpoN1 436861..438360 (-) 1500 WP_011000369.1 RNA polymerase factor sigma-54 Machinery gene
  ACEPM5_RS02070 (ACEPM5_02070) lptB 438458..439246 (-) 789 WP_011000370.1 LPS export ABC transporter ATP-binding protein -
  ACEPM5_RS02075 (ACEPM5_02075) lptA 439280..439855 (-) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEPM5_RS02080 (ACEPM5_02080) lptC 439924..440556 (-) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  ACEPM5_RS02085 (ACEPM5_02085) - 440558..441145 (-) 588 WP_011000373.1 HAD family hydrolase -
  ACEPM5_RS02090 (ACEPM5_02090) - 441184..442167 (-) 984 WP_016724265.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55227.56 Da        Isoelectric Point: 4.8064

>NTDB_id=1045294 ACEPM5_RS02065 WP_011000369.1 436861..438360(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6534]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1045294 ACEPM5_RS02065 WP_011000369.1 436861..438360(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN6534]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAACGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GJ62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

100

100

1

Multiple sequence alignment