Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J444_RS18105 Genome accession   NZ_CP007442
Coordinates   3648320..3648997 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli ACN001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3643320..3653997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J444_RS18070 (J444_3623) gspG 3643800..3644237 (+) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -
  J444_RS18075 (J444_3624) gspH 3644245..3644754 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  J444_RS18080 (J444_3625) gspI 3644751..3645128 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  J444_RS18085 (J444_3626) gspJ 3645121..3645708 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  J444_RS18090 (J444_3627) gspK 3645701..3646684 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  J444_RS18095 (J444_3628) gspL 3646699..3647862 (+) 1164 WP_001115230.1 type II secretion system protein GspL -
  J444_RS18100 (J444_3629) gspM 3647859..3648320 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  J444_RS18105 (J444_3630) pilD 3648320..3648997 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  J444_RS18110 (J444_3631) bfr 3649026..3649502 (-) 477 WP_000675504.1 bacterioferritin -
  J444_RS18115 (J444_3632) bfd 3649574..3649768 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J444_RS18120 (J444_3633) chiA 3649937..3652630 (-) 2694 WP_000773151.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=104484 J444_RS18105 WP_000178161.1 3648320..3648997(+) (pilD) [Escherichia coli ACN001]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=104484 J444_RS18105 WP_000178161.1 3648320..3648997(+) (pilD) [Escherichia coli ACN001]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment